ATP13A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ATP13A1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 16244-1-AP

Synonyms

KIAA1825, Endoplasmic reticulum transmembrane helix translocase, Endoplasmic reticulum P5A-ATPase, EC:7.4.2.-, CGI-152

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Validation Data Gallery

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  WB analysis using 16244-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16244-1-AP (ATP13A1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16244-1-AP

  IP result of anti-ATP13A1 (IP:16244-1-AP, 4ug; Detection:16244-1-AP 1:8000) with HeLa cells lysate 1560 ug.

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  IHC staining of mouse liver using 16244-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16244-1-AP (ATP13A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16244-1-AP

  Immunofluorescent analysis of HepG2 cells using 16244-1-AP (ATP13A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 12 publications below
• CoIP: See 1 publications below

Product Information

16244-1-AP targets ATP13A1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP13A1 fusion protein Ag9174
• Full Name: ATPase type 13A1
• Calculated Molecular Weight: 976 aa, 109 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC009302
• Gene Symbol: ATP13A1
• Gene ID (NCBI): 57130
• RRID: AB_2290293
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Science; The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.; Authors - Michael J McKenna ; KO Validated
human	WB	Mol Cell; Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology; Authors - Gayathri Muthukumar
human	WB	J Cell Sci; Mitochondrial antiviral-signalling protein is a client of the BAG6 protein quality control complex.; Authors - Peristera Roboti ; KD Validated
human	WB	J Biol Chem; The P5-type ATPase ATP13A1 modulates major histocompatibility complex I-related protein 1 (MR1)-mediated antigen presentation.; Authors - Corinna A Kulicke
human	WB	Cell Rep; P5A ATPase controls ER translocation of Wnt in neuronal migration.; Authors - Tingting Li
human	WB	Cell Rep; CATP-8/P5A ATPase Regulates ER Processing of the DMA-1 Receptor for Dendritic Branching.; Authors - Zhigang Feng