ATP13A1 Polyclonal antibody

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• ATP13A1 Polyclonal antibody (16244-1-AP)

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• KD/KO Validated

ATP13A1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16244-1-AP Copied!

Publications (14)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

ATP13A, CGI-152, EC:7.4.2.-, Endoplasmic reticulum P5A-ATPase, Endoplasmic reticulum transmembrane helix translocase

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  WB analysis using 16244-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16244-1-AP (ATP13A1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 16244-1-AP

  IP result of anti-ATP13A1 (IP:16244-1-AP, 4ug; Detection:16244-1-AP 1:8000) with HeLa cells lysate 1560 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse liver using 16244-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16244-1-AP (ATP13A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 16244-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16244-1-AP (ATP13A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse pancreas using 16244-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 16244-1-AP (ATP13A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse salivary gland using 16244-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse salivary gland tissue slide using 16244-1-AP (ATP13A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat pancreas using 16244-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 16244-1-AP (ATP13A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat salivary gland using 16244-1-AP

  Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 16244-1-AP (ATP13A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat stomach using 16244-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 16244-1-AP (ATP13A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 16244-1-AP

  Immunofluorescent analysis of HepG2 cells using 16244-1-AP (ATP13A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse liver tissue, rat pancreas tissue, mouse pancreas tissue, rat stomach tissue, mouse salivary gland tissue, human pancreas cancer tissue, rat salivary gland tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16244-1-AP targets ATP13A1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9174

  Product name: Recombinant human ATP13A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 615-758 aa of BC009302

  Sequence: QKEFVITSLKELGYVTLMCGDGTNDVGALKHADVGVALLANAPERVVERRRRPRDSPTLSNSGIRATSRTAKQRSGLPPSEEQPTSQRDRLSQVLRDLEDESTPIVKLGDASIAAPFTSKLSSIQCICHVIKQGRCTLVTTLQM

• Full Name: ATPase type 13A1
• Calculated Molecular Weight: 976 aa, 109 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC009302
• Gene Symbol: ATP13A1
• Gene ID (NCBI): 57130
• RRID: AB_2290293
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HD20
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP13A1 is a P-type ATPase, mainly involved in the transport of cations within cells. It plays an important role in maintaining intracellular ion homeostasis and membrane potential. ATP13A1 acts as a dislocase that mediates the ATP-dependent extraction of mislocalized mitochondrial transmembrane proteins from the endoplasmic reticulum membrane (PubMed:32973005).

Publications

What published studies show

The ATP13A1 Polyclonal antibody (Cat# 16244-1-AP) has 14 citations published in journals including Science, Molecular Cell, Advanced Science, Nature Structural & Molecular Biology, PNAS, Cell Reports, Journal of Biological Chemistry, Journal of Cell Science, and Life Science Alliance. Research fields span ER protein dislocation/topogenesis, mitochondrial outer membrane protein insertion, antiviral innate immunity (MAVS), Wnt signaling in neuronal migration, MHC antigen presentation, dendritic branching, protein quality control, and inflammation/tumor biology.

Automatically generated from the 14 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Science; The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.; Authors - Michael J McKenna ; KO Validated
human	WB	Science; MTCH2 is a mitochondrial outer membrane protein insertase; Authors - Alina Guna
human	WB,CoIP	Mol Cell; An ATP13A1-assisted topogenesis pathway for folding multi-spanning membrane proteins; Authors - Jia Ji ; KD Validated
human	WB	Mol Cell; ATP13A1 prevents ERAD of folding-competent mislocalized and misoriented proteins; Authors - Michael J McKenna
human	WB	Mol Cell; Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology; Authors - Gayathri Muthukumar
mouse,human	WB	Adv Sci (Weinh); The Endoplasmic Reticulum ATP13A1 is Essential for MAVS-Mediated Antiviral Innate Immunity; Authors - Rui Zhang ; KO Validated