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ATP1A1 Recombinant monoclonal antibody, PBS Only (83191-5-PBS)

Filter: AllIF/ICCFC (Intra)Affinity

IF Staining of A431 using 83191-5-RR (same clone as 83191-5-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1A1 antibody (83191-5-RR, Clone: 240160B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83191-5-PBS in a different storage buffer formulation.
IF Staining of A431 using 83191-5-RR (same clone as 83191-5-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1A1 antibody (83191-5-RR, Clone: 240160B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83191-5-PBS in a different storage buffer formulation.
FC experiment of HEK-293 using 83191-5-RR (same clone as 83191-5-PBS)
1x10^6 HEK-293 cells were intracellularly stained with 0.2 ug ATP1A1 Recombinant antibody (83191-5-RR, Clone:240160B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83191-5-PBS in a different storage buffer formulation.
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1x10^6 HEK-293 cells were intracellularly stained with 0.2 ug ATP1A1 Recombinant antibody (83191-5-RR, Clone:240160B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83191-5-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 83191-5-RR
Biolayer interferometry (BLl) kinetic assays of 83191-5-RR against Human ATP1A1 were performed. The affinity constant is 87.7 pM.

ATP1A1 Recombinant monoclonal antibody, PBS Only

Name: ATP1A1 Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 83191-5-PBS

ATP1A1 Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Cat No. 83191-5-PBS

Clone No.240160B5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

240160B5, ATP1A 1, Atpa 1, EC:7.2.2.13, Na(+)/K(+) ATPase 1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

83191-5-PBS targets ATP1A1 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag5763 Product name: Recombinant human ATP1A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 347-681 aa of BC050359 Sequence: TAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWLALSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIELCCGSVKEMRERYAKIVEIPFNSTNKYQLSIHKNPNTSEPQHLLVMKGAPERILDRCSSILLHGKEQPLDEELKDAFQNAYLELGGLGERVLGFCHLFLPDEQFPEGFQFDTDDVNFPIDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLDDI Predict reactive species
Full Name	ATPase, Na+/K+ transporting, alpha 1 polypeptide
Calculated Molecular Weight	113 kDa
GenBank Accession Number	BC050359
Gene Symbol	ATP1A1
Gene ID (NCBI)	476
ENSEMBL Gene ID	ENSG00000163399
RRID	AB_3670880
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	P05023
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein.