PMCA2 Polyclonal antibody

PMCA2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 19678-1-AP

Synonyms

ATP2B2, PMCA2, PMCA2a, PMCA2i

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Validation Data Gallery

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  WB analysis of human brain using 19678-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19678-1-AP (PMCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19678-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19678-1-AP (PMCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 19678-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19678-1-AP (PMCA2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 19678-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19678-1-AP (PMCA2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, HEK-293 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

19678-1-AP targets PMCA2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ATPase, Ca++ transporting, plasma membrane 2
• Calculated Molecular Weight: 139 kDa
• Observed Molecular Weight: 137 kDa
• GenBank Accession Number: NM_001001331
• Gene Symbol: PMCA2
• Gene ID (NCBI): 491
• RRID: AB_10644327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP2B2, also named as PMCA2, belongs to the cation transport ATPase (P-type) family and type IIB subfamily. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. ATG2B2 catalyze the reaction:ATP + H2O + Ca2+(Cis) = ADP + phosphate + Ca2+(Trans).

Publications

Species	Application	Title
human	WB	J Cell Sci; Rapid affinity purification of intracellular organelles using a twin strep tag.; Authors - Jian Xiong
chicken	WB	Metallomics; Thioredoxin silencing-induced cardiac supercontraction occurs through endoplasmic reticulum stress and calcium overload in chicken.; Authors - Jie Yang
	WB	Reprod Domest Anim; Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.; Authors - M D Xi
	WB	Mol Ther Nucleic Acids; lnc-3215 Suppression Leads to Calcium Overload in Selenium Deficiency-Induced Chicken Heart Lesion via the lnc-3215-miR-1594-TNN2 Pathway.; Authors - Jie Yang