ATP4B Polyclonal antibody

ATP4B Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18692-1-AP

Synonyms

Potassium-transporting ATPase subunit beta, H+/K+ ATPase beta, ATP4

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Validation Data Gallery

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  WB analysis using 18692-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18692-1-AP (ATP4B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 18692-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 18692-1-AP (ATP4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 18692-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 18692-1-AP (ATP4B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 18692-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 18692-1-AP (ATP4B antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse stomach using 18692-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using ATP4B antibody (18692-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse stomach tissue, mouse colon tissue, rat kidney tissue, rat stomach
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse stomach tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

18692-1-AP targets ATP4B in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP4B fusion protein Ag6935
• Full Name: ATPase, H+/K+ exchanging, beta polypeptide
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 60-80 kDa
• GenBank Accession Number: BC029059
• Gene Symbol: ATP4B
• Gene ID (NCBI): 496
• RRID: AB_10597241
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	FASEB J; Palmatine ameliorates N-methyl-N'-nitrosoguanidine-induced chronic atrophic gastritis through the STAT1/CXCL10 axis; Authors - Yuan Zhou