|Positive WB detected in||Jurkat cells, mouse liver tissue, rat brain tissue, rat liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human lung cancer tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17589-1-AP targets ATP5H in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATP5H fusion protein Ag11429|
|Full Name||ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d|
|Calculated molecular weight||137 aa, 16 kDa|
|Observed molecular weight||19-22 kDa|
|GenBank accession number||BC032245|
|Gene ID (NCBI)||10476|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H gene encodes ATP synthase subunit d of the Fo complex.
|Product Specific Protocols|
|WB protocol for ATP5H antibody 17589-1-AP||Download protocol|
|IHC protocol for ATP5H antibody 17589-1-AP||Download protocol|
|IF protocol for ATP5H antibody 17589-1-AP||Download protocol|
|IP protocol for ATP5H antibody 17589-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
SITING (Verified Customer) (05-25-2021)
Zee (Verified Customer) (01-28-2020)
It worked well with western blot.