ATP5H Polyclonal antibody

ATP5H Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (2)

Cat no : 17589-1-AP

Synonyms

My032, ATPQ, ATP5PD, ATP5JD, ATP5

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Validation Data Gallery

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  WB analysis of Jurkat using 17589-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17589-1-AP (ATP5H antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 17589-1-AP

  IP result of anti-ATP5H (IP:17589-1-AP, 3ug; Detection:17589-1-AP 1:2000) with mouse liver tissue lysate 6000ug.

• 

  IHC staining of human lung cancer using 17589-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17589-1-AP (ATP5H antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 17589-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17589-1-AP (ATP5H Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 17589-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17589-1-AP (ATP5H Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 17589-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17589-1-AP (ATP5H antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse liver tissue, rat brain tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human lung cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

17589-1-AP targets ATP5H in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP5H fusion protein Ag11429
• Full Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d
• Calculated Molecular Weight: 137 aa, 16 kDa
• Observed Molecular Weight: 19-22 kDa
• GenBank Accession Number: BC032245
• Gene Symbol: ATP5H
• Gene ID (NCBI): 10476
• RRID: AB_2062046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5H gene encodes ATP synthase subunit d of the Fo complex.

Publications

Species	Application	Title
human	WB	Brain; Ligand-free mitochondria-localized mutant AR-induced cytotoxicity in spinal bulbar muscular atrophy.; Authors - Xia Feng
mouse	WB	Theranostics; AdMSC-derived exosomes alleviate acute lung injury via transferring mitochondrial component to improve homeostasis of alveolar macrophages.; Authors - Liangjun Xia
human	IHC	Brain Pathol; Fatal familial insomnia: mitochondrial and protein synthesis machinery decline in the mediodorsal thalamus.; Authors - Margalida A Frau-Méndez
human	WB	Oncol Lett; Dihydroartemisinin inhibits liver cancer cell migration and invasion by reducing ATP synthase production through CaMKK2/NCLX; Authors - Jiang Chang
mouse	WB	J Nanobiotechnology; Modulation of alveolar macrophage and mitochondrial fitness by medicinal plant-derived nanovesicles to mitigate acute lung injury and viral pneumonia; Authors - Lusha Ye
human,mouse	WB	Science; Mitochondrial remodeling and ischemic protection by G protein-coupled receptor 35 agonists; Authors - Gregory A Wyant

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

SITING (Verified Customer) (05-25-2021)

good

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: EMBRYO HEART

ATP5H Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:17589-1-AP)

FH

Zee (Verified Customer) (01-28-2020)

It worked well with western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue extract