ATP5L Polyclonal antibody

ATP5L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16307-1-AP

Synonyms

ATP5JG, ATP5L, ATPase subunit g

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Validation Data Gallery

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  WB analysis using 16307-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16307-1-AP (ATP5L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

16307-1-AP targets ATP5L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP5L fusion protein Ag9287
• Full Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit G
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC015128
• Gene Symbol: ATP5L
• Gene ID (NCBI): 10632
• RRID: AB_10699871
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitochondrial membrane ATP synthase (F1-Fo ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. It is composed of the soluble catalytic core, F1, and the membrane-spanning component and Fo, which comprises the proton channel. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP5L gene encodes ATP synthase subunit g of the Fo complex.

Publications

Species	Application	Title
human		EBioMedicine; Mitochonic Acid 5 (MA-5) Facilitates ATP Synthase Oligomerization and Cell Survival in Various Mitochondrial Diseases.; Authors - Tetsuro Matsuhashi
rat	WB	J Proteome Res; Quantitative proteome of medulla oblongata in spontaneously hypertensive rats.; Authors - Sun Dongmei D
human	WB	PLoS One; Mic13 Is Essential for Formation of Crista Junctions in Mammalian Cells.; Authors - Ruchika Anand