ATP6AP1 Polyclonal antibody

ATP6AP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15305-1-AP

Synonyms

16A, Ac45, ATP6AP1, ATP6IP1, ATP6S1, CF2, Protein XAP 3, V ATPase Ac45 subunit, V ATPase S1 accessory protein, V ATPase subunit S1, VATPS1, XAP 3, XAP3

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Validation Data Gallery

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  WB analysis of mouse kidney using 15305-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15305-1-AP (ATP6AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15305-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15305-1-AP (ATP6AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15305-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15305-1-AP (ATP6AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 15305-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15305-1-AP (ATP6AP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15305-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15305-1-AP (ATP6AP1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HeLa cells, HepG2 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

15305-1-AP targets ATP6AP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP6AP1 fusion protein Ag7859
• Full Name: ATPase, H+ transporting, lysosomal accessory protein 1
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 46-52 kDa, 61-64 kDa
• GenBank Accession Number: BC000724
• Gene Symbol: ATP6AP1
• Gene ID (NCBI): 537
• RRID: AB_10804756
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Oncotarget; PMID: 28700346; Authors - Enrica Balza
human	WB	Proteomes; Integrative Proteomic and Phosphoproteomic Analyses of Hypoxia-Treated Pulmonary Artery Smooth Muscle Cells.; Authors - Ang Luo
human	IHC	Sci Rep; ATP6AP1 as a potential prognostic biomarker in CRC by comprehensive analysis and verification; Authors - Shijie Zhang