ATP6AP1 Polyclonal antibody

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  WB analysis of mouse kidney using 15305-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15305-1-AP (ATP6AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15305-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15305-1-AP (ATP6AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15305-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15305-1-AP (ATP6AP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 15305-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15305-1-AP (ATP6AP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15305-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15305-1-AP (ATP6AP1 Antibody) at dilution of 1:50 (under 40x lens).

ATP6AP1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15305-1-AP

Publications(5)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

ATP6S1, ATP6 S1, ATP6AP 1, Protein XAP-3, V-ATPase Ac45 subunit

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HeLa cells, HepG2 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

15305-1-AP targets ATP6AP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7859

  Product name: Recombinant human ATP6AP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 45-422 aa of BC000724

  Sequence: VPLVLWSSDRDLWAPAADTHEGHITSDLQLSTYLDPALELGPRNVLLFLQDKLSIEDFTAYGGVFGNKQDSAFSNLENALDLAPSSLVLPAVDWYAVSTLTTYLQEKLGASPLHVDLATLRELKLNASLPALLLIRLPYTASSGLMAPREVLTGNDEVIGQVLSTLKSEDVPYTAALTAVRPSRVARDVAVVAGGLGRQLLQKQPVSPVIHPPVSYNDTAPRILFWAQNFSVAYKDQWEDLTPLTFGVQELNLTGSFWNDSFARLSLTYERLFGTTVTFKFILANRLYPVSARHWFTMERLEVHSNGSVAYFNASQVTGPSIYSFHCEYVSSLSKKGSLLVARTQPSPWQMMLQDFQIQAFNVMGEQFSYASDCASFF

• Full Name: ATPase, H+ transporting, lysosomal accessory protein 1
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 46-52 kDa, 61-64 kDa
• GenBank Accession Number: BC000724
• Gene Symbol: ATP6AP1
• Gene ID (NCBI): 537
• RRID: AB_10804756
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15904
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Oncotarget; PMID: 28700346; Authors - Enrica Balza
human	WB	Proteomes; Integrative Proteomic and Phosphoproteomic Analyses of Hypoxia-Treated Pulmonary Artery Smooth Muscle Cells.; Authors - Ang Luo
human	IHC	Sci Rep; ATP6AP1 as a potential prognostic biomarker in CRC by comprehensive analysis and verification; Authors - Shijie Zhang
human	IHC	Cancer Cell Int; Lysosomal gene ATP6AP1 promotes doxorubicin resistance via up-regulating autophagic flux in breast cancer; Authors - Yinjiao Fei
rat	WB	Burns; Role of DLAT in cuproptosis and autophagy in hippocampal tissue of PTSD rats with high-voltage electrical burns; Authors - Chenyang Ge

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Celia (Verified Customer) (01-14-2025)

Great product, speedy delivery and easy order system with help from the team!

• Applications: Western Blot, Immunofluorescence