ATP6V0A1 Recombinant monoclonal antibody, PBS Only

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• ATP6V0A1 Recombinant monoclonal antibody, PBS Only (85596-4-PBS)

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  WB analysis using 85596-4-RR (same clone as 85596-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85596-4-RR (ATP6V0A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85596-4-PBS in a different storage buffer formulation.

ATP6V0A1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 85596-4-PBS

Clone No.243049G11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

a1, ATP6N1, ATP6N1A, Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit, Stv1

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Product Information

85596-4-PBS targets ATP6V0A1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4191

  Product name: Recombinant human ATP6V0A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-400 aa of BC032398

  Sequence: MGELFRSEEMTLAQLFLQSEAAYCCVSELGELGKVQFRDLNPDVNVFQRKFVNEVRRCEEMDRKLRFVEKEIRKANIPIMDTGENPEVPFPRDMIDLEANFEKIENELKEINTNQEALKRNFLELTELKFILRKTQQFFDEMADPDLLEESSSLLEPSEMGRGTPLRLGFVAGVINRERIPTFERMLWRVCRGNVFLRQAEIENPLEDPVTGDYVHKSVFIIFFQGDQLKNRVKKICEGFRASLYPCPETPQERKEMASGVNTRIDDLQMVLNQTEDHRQRVLQAAAKNIRVWFIKVRKMKAIYHTLNLCNIDVTQKCLIAEVWCPVTDLDSIQFALRRGTEHSGSTVPSILNRMQTNQTPPTYNKTNKFTYGFQNIVDAYGIGTYREINPAPYTIITFP

• Full Name: ATPase, H+ transporting, lysosomal V0 subunit a1
• Calculated Molecular Weight: 837 aa, 96 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC032398
• Gene Symbol: ATP6V0A1
• Gene ID (NCBI): 535
• RRID: AB_3744226
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q93050
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation(PMID: 7774924 34904569).