ATP6V0A4 Polyclonal antibody

• Featured Product
• KD/KO Validated

ATP6V0A4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 21570-1-AP

Synonyms

A4, ATP6N1B, ATP6N2, ATP6V0A4, RDRTA2, RTA1C, RTADR, STV1, V ATPase 116 kDa isoform a4, VPH1, VPP2

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Validation Data Gallery

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  WB analysis using 21570-1-AP

  Mouse kidney lysates were subjected to SDS PAGE followed by western blot with 21570-1-AP (ATP6V0A4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 21570-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21570-1-AP (ATP6V0A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

The immunogen of 21570-1-AP is ATP6V0A4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATP6V0A4 fusion protein Ag16095
• Full Name: ATPase, H+ transporting, lysosomal V0 subunit a4
• Calculated Molecular Weight: 840 aa, 96 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC109305
• Gene Symbol: ATP6V0A4
• Gene ID (NCBI): 50617
• RRID: AB_2918072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ATP6V0A4 is a component of vacuolar-H+ATPase (V-ATPase) which is a multi-subunit enzyme that couples ATP hydrolysis to proton pumping across membranes. The V-ATPases are comprised of two major parts, the cytosolic V1 domain involved in ATP-binding and subsequent hydrolysis, and the membrane-associated V0 domain responsible for proton translocation. The V0 domain is composed of five subunits: a, c, c', c'' and d. The 'a' subunit of V0 domain has four isoforms : a1-a4. It has been found that mutations in ATP6V0A4(a4) are associated with distal renal tubular acidosis(dRTA) combined in some cases with progressive hearing loss leading to sensorineural deafness. This antibody was generated against the internal region of human ATP6V0A4 and is predicted to detect the a4 isoform only.

Publications

Species	Application	Title
human	IHC	Oncol Lett; Identification of ATP6V0A4 as a potential biomarker in renal cell carcinoma using integrated bioinformatics analysis; Authors - Jinming Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christopher (Verified Customer) (12-14-2020)

We used the anti-ATP6V0A4 antibody. In lysates of homozygous Atp6v0a4-KO mice (described in Hennings et al., EMBO Mol Med, 2012) the signal for ATP6V0A4 was not detected anymore. Thus, the antibody is specific and does not cross-react.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Kidney lysate, IN-IC and HK-2 cells

ATP6V0A4 Antibody Western Blot validation (1:1000 dilution) in Kidney lysate, IN-IC and HK-2 cells (Cat no:21570-1-AP)