ATP6V1A Polyclonal antibody

ATP6V1A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 51152-1-AP


ATP6A1, ATP6V1A, ATP6V1A1, HO68, V ATPase 69 kDa subunit, V ATPase subunit A, VA68, Vacuolar ATPase isoform VA68, Vma1, VPP2

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, human brain tissue, mouse kidney tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51152-1-AP targets ATP6V1A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberNM_001690
Gene Symbol ATP6V1A
Gene ID (NCBI) 523
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.This antibody is specific to ATP6V1A.


Product Specific Protocols
WB protocol for ATP6V1A antibody 51152-1-APDownload protocol
IHC protocol for ATP6V1A antibody 51152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols