ATP6V1B1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 83953-5-RR (same clone as 83953-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83953-5-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 83953-5-RR (same clone as 83953-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83953-5-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 83953-5-RR (same clone as 83953-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83953-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 83953-5-RR (same clone as 83953-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83953-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 83953-5-RR (same clone as 83953-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83953-5-RR (ATP6V1B1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83953-5-PBS in a different storage buffer formulation.

ATP6V1B1 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 83953-5-PBS

Clone No.240955B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

V-type proton ATPase subunit B, kidney isoform, VPP3, V-ATPase subunit B 1, VATB, ATP6B1

---

Product Information

83953-5-PBS targets ATP6V1B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6332

  Product name: Recombinant human ATP6V1B1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-327 aa of BC063411

  Sequence: MAMEIDSRPGGLPGSSCNLGAAREHMQAVTRNYITHPRVTYRTVCSVNGPLVVLDRVKFAQYAEIVHFTLPDGTQRSGQVLEVAGTKAIVQVFEGTSGIDARKTTCEFTGDILRTPVSEDMLGRVFNGSGKPIDKGPVVMAEDFLDINGQPINPHSRIYPEEMIQTGISPIDVMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKAVLDYHDDNFAIVFAAMGVNMETARFFKSDFEQNGTMGNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLAT

• Full Name: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC063411
• Gene Symbol: ATP6V1B1
• Gene ID (NCBI): 525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P15313
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ATP6V1B1, also named ATP6B1, VATB and VPP3, belongs to the ATPase alpha/beta chains family. ATP6V1B1 is mainly expressed in kidney. ATP6V1B1 is essential for the proper assembly and activity of V-ATPase. In renal intercalated cells, ATP6V1B1 mediates secretion of protons (H+) into the urine thereby ensuring correct urinary acidification. The calculated molecular weight of ATP6V1B1 is 57 kDa.