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ATP6V1C2 Polyclonal antibody

ATP6V1C2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

ATP6C2, V-ATPase subunit C 2, VMA5, V-type proton ATPase subunit C 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 
SKU: 

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Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inmouse kidney tissue, human kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16274-1-AP targets ATP6V1C2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9349

Product name: Recombinant human ATP6V1C2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-381 aa of BC012142

Sequence: MSEFWLISAPGDKENLQALERMNTVTSKSNLSYNTKFAIPDFKVGTLDSLVGLSDELGKLDTFAESLIRRMAQSVVEVMEDSKGKVQEHLLANGVDLTSFVTHFEWDMAKYPVKQPLVSVVDTIAKQLAQIEMDLKSRTAAYDTLKTNLENLEKKSMGNLFTRTLSDIVSKEDFVLDSEYLVTLLVIVPKPNYSQWQKTYESLSDMVVPRSTKLITEDKEGGLFTVTLFRKVIEDFKTKAKENKFTVREFYYDEKEIEREREEMARLLSDKKQQYGPLLRWLKVNFSEAFIAWIHIKALRVFVESVLRYGLPVNFQAVLLQPHKKSSTKRLREVLNSVFRHLDEVAATSILDASVEIPGLQLNNQDYFPYVYFHIDLSLLD

Predict reactive species
Full Name ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C2
Calculated Molecular Weight 381 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC012142
Gene Symbol ATP6V1C2
Gene ID (NCBI) 245973
RRIDAB_2062522
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NEY4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for ATP6V1C2 antibody 16274-1-APDownload protocol
WB protocol for ATP6V1C2 antibody 16274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The ATP6V1C2 polyclonal antibody (Cat# 16274-1-AP) has 4 total citations. These appear in Nature Communications, Environmental Health Perspectives, Pathology Research and Practice, and Frontiers in Genetics. The research fields span lysosomal dysfunction and ferroptosis, inorganic arsenic effects on prostate stem-progenitor cell transformation and NRF2 pathway activation, prognostic value of ATP6V1C2 in colorectal cancer, and survival prediction in colon adenocarcinoma.

SpeciesApplicationTitle
WB

Nat Commun

Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis

Authors - Tze Mun Loo
humanWB,IF

Environ Health Perspect

Effects of Inorganic Arsenic on Human Prostate Stem-Progenitor Cell Transformation, Autophagic Flux Blockade, and NRF2 Pathway Activation.

Authors - Lishi Xie
  • KD Validated
humanWB

Pathol Res Pract

Comprehensive analysis of ATP6V1s family member, ATP6V1C2, with prognostic and drug development values in colorectal cancer

Authors - Jun Li
humanWB,IHC

Front Genet

High Expression of ATP6V1C2 Predicts Unfavorable Overall Survival in Patients With Colon Adenocarcinoma

Authors - Guanghua Li
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