Tested Applications
| Positive WB detected in | mouse kidney tissue, human kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16274-1-AP targets ATP6V1C2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9349 Product name: Recombinant human ATP6V1C2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-381 aa of BC012142 Sequence: MSEFWLISAPGDKENLQALERMNTVTSKSNLSYNTKFAIPDFKVGTLDSLVGLSDELGKLDTFAESLIRRMAQSVVEVMEDSKGKVQEHLLANGVDLTSFVTHFEWDMAKYPVKQPLVSVVDTIAKQLAQIEMDLKSRTAAYDTLKTNLENLEKKSMGNLFTRTLSDIVSKEDFVLDSEYLVTLLVIVPKPNYSQWQKTYESLSDMVVPRSTKLITEDKEGGLFTVTLFRKVIEDFKTKAKENKFTVREFYYDEKEIEREREEMARLLSDKKQQYGPLLRWLKVNFSEAFIAWIHIKALRVFVESVLRYGLPVNFQAVLLQPHKKSSTKRLREVLNSVFRHLDEVAATSILDASVEIPGLQLNNQDYFPYVYFHIDLSLLD Predict reactive species |
| Full Name | ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C2 |
| Calculated Molecular Weight | 381 aa, 44 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC012142 |
| Gene Symbol | ATP6V1C2 |
| Gene ID (NCBI) | 245973 |
| RRID | AB_2062522 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NEY4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ATP6V1C2 antibody 16274-1-AP | Download protocol |
| WB protocol for ATP6V1C2 antibody 16274-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ATP6V1C2 polyclonal antibody (Cat# 16274-1-AP) has 4 total citations. These appear in Nature Communications, Environmental Health Perspectives, Pathology Research and Practice, and Frontiers in Genetics. The research fields span lysosomal dysfunction and ferroptosis, inorganic arsenic effects on prostate stem-progenitor cell transformation and NRF2 pathway activation, prognostic value of ATP6V1C2 in colorectal cancer, and survival prediction in colon adenocarcinoma.
| Species | Application | Title |
|---|---|---|
Nat Commun Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis | ||
Environ Health Perspect Effects of Inorganic Arsenic on Human Prostate Stem-Progenitor Cell Transformation, Autophagic Flux Blockade, and NRF2 Pathway Activation.
| ||
Pathol Res Pract Comprehensive analysis of ATP6V1s family member, ATP6V1C2, with prognostic and drug development values in colorectal cancer | ||
Front Genet High Expression of ATP6V1C2 Predicts Unfavorable Overall Survival in Patients With Colon Adenocarcinoma |













