ATP6V1H Polyclonal antibody

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• ATP6V1H Polyclonal antibody (26683-1-AP)

ATP6V1H Polyclonal Antibody for WB, IP, ELISA

Cat No. 26683-1-AP Copied!

Publications (16)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

ATP6V1H, CGI 11, MSTP042, NBP1, Nef binding protein 1, SFD, SFDalpha, SFDbeta, V ATPase 50/57 kDa subunits, V ATPase subunit H, V type proton ATPase subunit H, Vacuolar proton pump subunit H, VMA13

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  WB analysis of mouse brain using 26683-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26683-1-AP (ATP6V1H Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat brain using 26683-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 26683-1-AP (ATP6V1H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 26683-1-AP

  IP result of anti-ATP6V1H (IP:26683-1-AP, 4ug; Detection:26683-1-AP 1:300) with mouse brain tissue lysate 3000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26683-1-AP targets ATP6V1H in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24688

  Product name: Recombinant human ATP6V1H protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 296-483 aa of BC025275

  Sequence: STERETRQEYALAMIQCKVLKQLENLEQQKYDDEDISEDIKFLLEKLGESVQDLSSFDEYSSELKSGRLEWSPVHKSEKFWRENAVRLNEKNYELLKILTKLLEVSDDPQVLAVAAHDVGEYVRHYPRGKRVIEQLGGKQLVMNHMHHEDQQVRYNALLAVQKLMVHNWEYLGKQLQSEQPQTAAARS

• Full Name: ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H
• Calculated Molecular Weight: 483 aa, 56 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC025275
• Gene Symbol: ATP6V1H
• Gene ID (NCBI): 51606
• RRID: AB_2880601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UI12
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains.

Publications

What published studies show

The ATP6V1H polyclonal antibody (Cat# 26683-1-AP) has 16 citations across journals including Nature Communications, Molecular Cell, Cell Metabolism, Autophagy, Nature Structural & Molecular Biology, Developmental Cell, Frontiers in Immunology, International Journal of Molecular Sciences, Molecular Oncology, Journal of Cellular Physiology, Biochemical and Biophysical Research Communications, and Biophysical Reports. Research fields encompass V-ATPase structure and function, lysosomal biology, autophagy, innate immunity, glioblastoma, bone loss, hepatic injury, ferroptosis, and insulin secretion.

Automatically generated from the 16 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse		Cell Metab; ABCG2 is an itaconate exporter that limits antibacterial innate immunity by alleviating TFEB-dependent lysosomal biogenesis; Authors - Chao Chen
human	WB	Autophagy; A CRISPR-Cas9 screen identifies LAPTM4A (lysosomal protein transmembrane 4 alpha) as a key host barrier against PRRSV infection.; Authors - Zhan He
human	WB	Autophagy; YWHA/14-3-3 proteins recognize phosphorylated TFEB by a noncanonical mode for controlling TFEB cytoplasmic localization.; Authors - Yang Xu
human	WB	Autophagy; Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate; Authors - Mengting Ou
		Nat Commun; The V-ATPase/ATG16L1 axis drives membrane remodeling during epithelial morphogenesis.; Authors - Gabriel Baonza
		Nat Commun; The V-ATPase/ATG16L1 axis drives membrane remodeling during epithelial morphogenesis.; Authors - Gabriel Baonza