ATP9A Polyclonal antibody

ATP9A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 19504-1-AP

Synonyms

Probable phospholipid-transporting ATPase IIA, KIAA0611, EC:7.6.2.1, ATPIIA

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Validation Data Gallery

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  WB analysis of mouse spleen using 19504-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19504-1-AP (ATP9A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 19504-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 19504-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat brain using 19504-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of rat brain using 19504-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 19504-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19504-1-AP (ATP9A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse spleen tissue
• Positive IHC detected in: human testis tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19504-1-AP targets ATP9A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ATPase, class II, type 9A
• Calculated Molecular Weight: 119 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: NM_006045
• Gene Symbol: ATP9A
• Gene ID (NCBI): 10079
• RRID: AB_10640806
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP9A, also named as ATPIIA and KIAA0611, belongs to the cation transport ATPase (P-type) family and Type IV subfamily. ATP9A catalyzes the reaction: ATP + H2O + phospholipid(In) = ADP + phosphate + phospholipid(Out).

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (06-16-2019)

It recognizes ATP9A (top most, prominent band) but also quite a few non-specific ones.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

ATP9A Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:19504-1-AP)