Tested Applications
| Positive WB detected in | mouse spleen tissue |
| Positive IHC detected in | human testis tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19504-1-AP targets ATP9A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ATPase, class II, type 9A |
| Calculated Molecular Weight | 119 kDa |
| Observed Molecular Weight | 105 kDa |
| GenBank Accession Number | NM_006045 |
| Gene Symbol | ATP9A |
| Gene ID (NCBI) | 10079 |
| RRID | AB_10640806 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75110 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATP9A, also named as ATPIIA and KIAA0611, belongs to the cation transport ATPase (P-type) family and Type IV subfamily. ATP9A catalyzes the reaction: ATP + H2O + phospholipid(In) = ADP + phosphate + phospholipid(Out).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATP9A antibody 19504-1-AP | Download protocol |
| IHC protocol for ATP9A antibody 19504-1-AP | Download protocol |
| WB protocol for ATP9A antibody 19504-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this antibody (used in Western Blot at a 1:1,000 dilution on MDCK GII cells) gave it a 4-star rating. The reviewer noted that it successfully recognizes ATP9A, producing a prominent top band, but also generates a number of non-specific bands. Overall, the antibody performs well for its target but may require optimization to reduce background.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Joleen (Verified Customer) (06-16-2019) | It recognizes ATP9A (top most, prominent band) but also quite a few non-specific ones.
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