ATP9A Polyclonal antibody

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• ATP9A Polyclonal antibody (19504-1-AP)

ATP9A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19504-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Probable phospholipid-transporting ATPase IIA, KIAA0611, EC:7.6.2.1, ATPIIA

• 

  WB analysis of mouse spleen using 19504-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19504-1-AP (ATP9A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human testis using 19504-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human testis using 19504-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat brain using 19504-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat brain using 19504-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19504-1-AP (ATP9A Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 19504-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19504-1-AP (ATP9A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse spleen tissue
• Positive IHC detected in: human testis tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19504-1-AP targets ATP9A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: ATPase, class II, type 9A
• Calculated Molecular Weight: 119 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: NM_006045
• Gene Symbol: ATP9A
• Gene ID (NCBI): 10079
• RRID: AB_10640806
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75110
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP9A, also named as ATPIIA and KIAA0611, belongs to the cation transport ATPase (P-type) family and Type IV subfamily. ATP9A catalyzes the reaction: ATP + H2O + phospholipid(In) = ADP + phosphate + phospholipid(Out).

Reviews

4.0 out of 5

1 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Joleen (Verified Customer) (06-16-2019)

It recognizes ATP9A (top most, prominent band) but also quite a few non-specific ones.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

ATP9A Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:19504-1-AP)