CARNS1 Polyclonal antibody

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• CARNS1 Polyclonal antibody (25725-1-AP)

CARNS1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25725-1-AP Copied!

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ATPGD1, ATP grasp domain containing 1, ATP-grasp domain-containing protein 1, Carnosine synthase 1, EC:6.3.2.11

• 

  WB analysis using 25725-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25725-1-AP (CARNS1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse liver using 25725-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25725-1-AP (CARNS1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse liver using 25725-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25725-1-AP (CARNS1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25725-1-AP targets CARNS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22812

  Product name: Recombinant human ATPGD1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 443-827 aa of BC036557

  Sequence: MTEHRRDEENARLLAELVRARGLKLDGCFSYWDDCLVLTALLCQELGLPCSSPAALRLAKQKSLTQLHLLHHHGPPWPAPSLHAVPCCPLESEADVERAVHQVPLPGVMKLEFGAGAVGVRLVEDAPQCHEHFSRITRDLQGEADHPGIGLGWGNAMLLMEFVEGTEHDVDLVLFGGRLLAAFVSDNGPTRLPGFTETAACMPTGLAPEQEAQMVQAAFRCCLGCGLLDGVFNVELKLTGAGPRLIEINPRMGGFYLRDWILELYGVDLLLAAVMVACGLRPALPTRPRARGHLVGVMCLVSQHLQALSSTASRETLQALHDRGLLRLNLLEEALVPGEYEEPYCSVACAGPSPTEARLRLLGLCQGLGIDGPSYPVAHFLSHFK

• Full Name: ATP-grasp domain containing 1
• Calculated Molecular Weight: 827 aa, 88 kDa
• Observed Molecular Weight: 88-103 kDa
• GenBank Accession Number: BC036557
• Gene Symbol: CARNS1
• Gene ID (NCBI): 57571
• RRID: AB_2880211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A5YM72
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CARNS1, located at human chromosome 11q13.2, encodes carnosine synthase 1 belonging to the ATP-grasp ATPase family (EC 6.3.2.11). It mediates ATP-dependent synthesis of carnosine in skeletal muscle and homocarnosine in the central nervous system, two vital histidine-containing dipeptides with antioxidant and pH-buffering effects. This gene is highly conserved across vertebrates and preferentially expressed in brain and muscle tissues. Aberrant CARNS1 expression disturbs dipeptide homeostasis, linking to aging-related sensory decline and tumor progression via impaired carnosine-mediated tumor suppression. (PMID: 20097752)

Publications

What published studies show

The CARNS1 Polyclonal antibody (Cat# 25725-1-AP) has 5 total citations. These appear in Autophagy, Redox Biology, Particulate and Fibre Toxicology, Neurochemistry Research, and Translational Stroke Research. The associated research fields include mitophagy and axon regeneration, oxidative stress and necroptosis in asthma, vascular toxicity from particulate matter, liver failure, and post-cerebral ischemic hypoperfusion.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IHC	Autophagy; FUNDC1-dependent mitophagy determines axon regeneration capacity.; Authors - Wenlei Li
		Redox Biol; Histidine metabolic reprogramming drives oxidative stress induced mtDNA release to promote necroptosis and airway inflammation in severe asthma.; Authors - Ying Chen
	IHC	Part Fibre Toxicol; Aryl hydrocarbon receptor activation-mediated vascular toxicity of ambient fine particulate matter: contribution of polycyclic aromatic hydrocarbons and osteopontin as a biomarker.; Authors - Chia-Chi Ho
rat	WB	Neurochem Res; Carnosine Reduces Oxidative Stress and Reverses Attenuation of Righting and Postural Reflexes in Rats with Thioacetamide-Induced Liver Failure.; Authors - Krzysztof Milewski
rat	WB	Transl Stroke Res; Nicotine Exposure Along with Oral Contraceptive Treatment in Female Rats Exacerbates Post-cerebral Ischemic Hypoperfusion Potentially via Altered Histamine Metabolism.; Authors - Nathan d'Adesky