CARNS1 Recombinant monoclonal antibody

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• CARNS1 Recombinant monoclonal antibody (88342-1-RR)

CARNS1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 88342-1-RR

Clone No.260860B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ATPGD1, ATP grasp domain containing 1, ATP-grasp domain-containing protein 1, Carnosine synthase 1, EC:6.3.2.11

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  WB analysis using 88342-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88342-1-RR (ATPGD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: pig skeletal muscle tissue, human skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88342-1-RR targets CARNS1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag22761

  Product name: Recombinant human ATPGD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 443-827 aa of BC036557

  Sequence: MTEHRRDEENARLLAELVRARGLKLDGCFSYWDDCLVLTALLCQELGLPCSSPAALRLAKQKSLTQLHLLHHHGPPWPAPSLHAVPCCPLESEADVERAVHQVPLPGVMKLEFGAGAVGVRLVEDAPQCHEHFSRITRDLQGEADHPGIGLGWGNAMLLMEFVEGTEHDVDLVLFGGRLLAAFVSDNGPTRLPGFTETAACMPTGLAPEQEAQMVQAAFRCCLGCGLLDGVFNVELKLTGAGPRLIEINPRMGGFYLRDWILELYGVDLLLAAVMVACGLRPALPTRPRARGHLVGVMCLVSQHLQALSSTASRETLQALHDRGLLRLNLLEEALVPGEYEEPYCSVACAGPSPTEARLRLLGLCQGLGIDGPSYPVAHFLSHFK

• Full Name: ATP-grasp domain containing 1
• Calculated Molecular Weight: 827 aa, 88 kDa
• Observed Molecular Weight: 88-103 kDa
• GenBank Accession Number: BC036557
• Gene Symbol: CARNS1
• Gene ID (NCBI): 57571
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: A5YM72
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CARNS1, located at human chromosome 11q13.2, encodes carnosine synthase 1 belonging to the ATP-grasp ATPase family (EC 6.3.2.11). It mediates ATP-dependent synthesis of carnosine in skeletal muscle and homocarnosine in the central nervous system, two vital histidine-containing dipeptides with antioxidant and pH-buffering effects. This gene is highly conserved across vertebrates and preferentially expressed in brain and muscle tissues. Aberrant CARNS1 expression disturbs dipeptide homeostasis, linking to aging-related sensory decline and tumor progression via impaired carnosine-mediated tumor suppression. (PMID: 20097752)