ATRIP Polyclonal antibody

• Featured Product
• KD/KO Validated

ATRIP Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11327-1-AP

Synonyms

AGS1, ATR interacting protein, ATRIP

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Validation Data Gallery

• 

  WB analysis of MCF-7 using 11327-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11327-1-AP (ATRIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 11327-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11327-1-AP (ATRIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11327-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11327-1-AP (ATRIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11327-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11327-1-AP (ATRIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11327-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11327-1-AP (ATRIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 11327-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11327-1-AP (ATRIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 11327-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11327-1-AP (ATRIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 11327-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11327-1-AP (ATRIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 11327-1-AP

  IP Result of anti-ATRIP (IP:11327-1-AP, 3ug; Detection:11327-1-AP 1:1000) with MCF-7 cells lysate 2800ug.

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Tested Applications

• Positive WB detected in: MCF7 cells, A549 cells, BxPC-3 cells, HeLa cells, NIH/3T3 cells, PC-3 cells
• Positive IP detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

11327-1-AP targets ATRIP in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATRIP fusion protein Ag1869
• Full Name: ATR interacting protein
• Calculated molecular weight: 791 aa, 86 kDa
• Observed molecular weight: 86-90 kDa
• GenBank accession number: BC014153
• Gene symbol: ATRIP
• Gene ID (NCBI): 84126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IHC	Biomaterials; A fluorinated peptide with high serum- and lipid-tolerence for the delivery of siRNA drugs to treat obesity and metabolic dysfunction.; Authors - Jin Qiu ; KD Validated
mouse	WB	Biochem J; Mutation of the RelA(p65) Thr505 phosphosite disrupts the DNA replication stress response leading to CHK1 inhibitor resistance; Authors - Jill E Hunter
mouse	WB	Biochem J; Regulation of CHK1 inhibitor resistance by a c-Rel and USP1 dependent pathway; Authors - Jill E Hunter