|Positive WB detected in||Jurkat cells, mouse thymus tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human tonsillitis tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 24822-1-AP is ATXN2L Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATXN2L fusion protein Ag20581|
|Full Name||ataxin 2-like|
|Calculated molecular weight||1062 aa, 112 kDa|
|Observed molecular weight||~140 kDa|
|GenBank accession number||BC082760|
|Gene ID (NCBI)||11273|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sahil (Verified Customer) (06-21-2019)
I have checked others too and this works perfectly fine. If needed again would definitely buy it.