ATXN2L Polyclonal antibody

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  WB analysis using 24822-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24822-1-AP (ATXN2L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24822-1-AP (ATXN2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24822-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24822-1-AP (ATXN2L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24822-1-AP

  IP result of anti-ATXN2L (IP:24822-1-AP, 4ug; Detection:24822-1-AP 1:2000) with HeLa cells lysate 2400ug.

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  IHC staining of human tonsillitis using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24822-1-AP (ATXN2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24822-1-AP (ATXN2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24822-1-AP (ATXN2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24822-1-AP (ATXN2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24822-1-AP (ATXN2L Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24822-1-AP (ATXN2L Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 24822-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ATXN2L antibody (24822-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

ATXN2L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 24822-1-AP

Publications(9)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Ataxin-2 domain protein, ataxin 2 like, A2RP, A2LP, A2LG

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, MKN-45 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human tonsillitis tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

24822-1-AP targets ATXN2L in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATXN2L fusion protein Ag20581
• Full Name: ataxin 2-like
• Calculated Molecular Weight: 1062 aa, 112 kDa
• Observed Molecular Weight: ~140 kDa
• GenBank Accession Number: BC082760
• Gene Symbol: ATXN2L
• Gene ID (NCBI): 11273
• RRID: AB_2879743
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WWM7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATXN2L is a paralog of Ataxin-2 (ATXN2) but without abnormal polyQ expended track, which is conserved in most of the ATXNs and drives the pathogenesis of neurodegeneration. It was reported that ATXN2L was widely expressed in immortalized cell lines, and ATXN2L-JAK2 fusion was found in CD4-positive T-cell lymphoma.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,CoIP	Cell; Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules; Authors - Qinqin Cui
mouse	IF,WB	Cell; Circadian clocks are modulated by compartmentalized oscillating translation; Authors - Yanrong Zhuang
mouse	WB	Int J Mol Sci; Mid-Gestation lethality of Atxn2l-Ablated Mice.; Authors - Jana Key
human	WB	ACS Chem Biol; Deorphanizing Caspase-3 and Caspase-9 Substrates In and Out of Apoptosis with Deep Substrate Profiling.; Authors - Luam E Araya
rat	WB	Phytother Res; Jatrorrhizine ameliorates Schwann cell myelination via inhibiting HDAC3 ability to recruit Atxn2l for regulating the NRG1-ErbB2-PI3K-AKT pathway in diabetic peripheral neuropathy mice; Authors - Xiaobao Gong
human	WB	Cell Genom; Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders; Authors - Greta Pintacuda

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sahil (Verified Customer) (06-21-2019)

I have checked others too and this works perfectly fine. If needed again would definitely buy it.

• Applications: Western Blot,