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ATXN2L Polyclonal antibody

ATXN2L Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 24822-1-AP

Synonyms

A2D, A2LG, A2LP, A2RP, Ataxin 2 domain protein, ataxin 2 like, Ataxin 2 like protein, Ataxin 2 related protein, ATXN2L



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, MKN-45 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman tonsillitis tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

24822-1-AP targets ATXN2L in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATXN2L fusion protein Ag20581
Full Name ataxin 2-like
Calculated molecular weight 1062 aa, 112 kDa
Observed molecular weight ~140 kDa
GenBank accession numberBC082760
Gene symbol ATXN2L
Gene ID (NCBI) 11273
RRIDAB_2879743
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATXN2L is a paralog of Ataxin-2 (ATXN2) but without abnormal polyQ expended track, which is conserved in most of the ATXNs and drives the pathogenesis of neurodegeneration. It was reported that ATXN2L was widely expressed in immortalized cell lines, and ATXN2L-JAK2 fusion was found in CD4-positive T-cell lymphoma.

Protocols

Product Specific Protocols
WB protocol for ATXN2L antibody 24822-1-APDownload protocol
IHC protocol for ATXN2L antibody 24822-1-APDownload protocol
IF protocol for ATXN2L antibody 24822-1-APDownload protocol
IP protocol for ATXN2L antibody 24822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF,WB

Cell

Circadian clocks are modulated by compartmentalized oscillating translation

Authors - Yanrong Zhuang
mouseWB

Int J Mol Sci

Mid-Gestation lethality of Atxn2l-Ablated Mice.

Authors - Jana Key
humanWB

ACS Chem Biol

Deorphanizing Caspase-3 and Caspase-9 Substrates In and Out of Apoptosis with Deep Substrate Profiling.

Authors - Luam E Araya
ratWB

Phytother Res

Jatrorrhizine ameliorates Schwann cell myelination via inhibiting HDAC3 ability to recruit Atxn2l for regulating the NRG1-ErbB2-PI3K-AKT pathway in diabetic peripheral neuropathy mice

Authors - Xiaobao Gong
humanWB,CoIP

Cell

Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules

Authors - Qinqin Cui
humanWB

Cell Genom

Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders

Authors - Greta Pintacuda

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sahil (Verified Customer) (06-21-2019)

I have checked others too and this works perfectly fine. If needed again would definitely buy it.

  • Applications: Western Blot,