ATXN2L PolyClonal antibody

• Featured Product
• KD/KO Validated

ATXN2L PolyClonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 24822-1-AP

Synonyms

A2D, A2LG, A2LP, A2RP, Ataxin 2 domain protein, ataxin 2 like, Ataxin 2 like protein, Ataxin 2 related protein, ATXN2L

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Validation Data Gallery

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  WB analysis using 24822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24822-1-AP (ATXN2L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 24822-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 24822-1-AP (ATXN2L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 24822-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24822-1-AP (ATXN2L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 24822-1-AP

  IP Result of anti-ATXN2L (IP:24822-1-AP, 4ug; Detection:24822-1-AP 1:2000) with HeLa cells lysate 2400ug.

• 

  IHC staining of human tonsillitis using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24822-1-AP (ATXN2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24822-1-AP (ATXN2L Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24822-1-AP (ATXN2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24822-1-AP (ATXN2L Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24822-1-AP (ATXN2L Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24822-1-AP (ATXN2L Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24822-1-AP (ATXN2L Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 24822-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24822-1-AP (ATXN2L Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse thymus tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human tonsillitis tissue, human testis tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

The immunogen of 24822-1-AP is ATXN2L Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: PolyClonal
• Type: Antibody
• Immunogen: ATXN2L fusion protein Ag20581
• Full Name: ataxin 2-like
• Calculated molecular weight: 1062 aa, 112 kDa
• Observed molecular weight: ~140 kDa
• GenBank accession number: BC082760
• Gene symbol: ATXN2L
• Gene ID (NCBI): 11273
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB	Cell Death Dis; PMID: 30787271; Authors - Li Lin ; KD Validated
mouse	WB	Int J Mol Sci; Mid-Gestation lethality of Atxn2l-Ablated Mice.; Authors - Jana Key
human	WB	ACS Chem Biol; Deorphanizing Caspase-3 and Caspase-9 Substrates In and Out of Apoptosis with Deep Substrate Profiling.; Authors - Luam E Araya

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sahil (Verified Customer) (06-21-2019)

I have checked others too and this works perfectly fine. If needed again would definitely buy it.

• Applications: Western Blot,

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