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ATXN2L PolyClonal antibody

ATXN2L PolyClonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24822-1-AP

Synonyms

A2D, A2LG, A2LP, A2RP, Ataxin 2 domain protein, ataxin 2 like, Ataxin 2 like protein, Ataxin 2 related protein, ATXN2L

Validation Data Gallery

  • WB analysis of Jurkat using 24822-1-AP

    WB analysis of Jurkat using 24822-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24822-1-AP (ATXN2L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 24822-1-AP

    IP experiment of HeLa using 24822-1-AP

    IP Result of anti-ATXN2L (IP:24822-1-AP, 4ug; Detection:24822-1-AP 1:2000) with HeLa cells lysate 2400ug.

  • IHC staining of human tonsillitis using 24822-1-AP

    IHC staining of human tonsillitis using 24822-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24822-1-AP (ATXN2L Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human testis using 24822-1-AP

    IHC staining of human testis using 24822-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24822-1-AP (ATXN2L Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inJurkat cells, mouse thymus tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman tonsillitis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24822-1-AP is ATXN2L Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class PolyClonal
Type Antibody
Immunogen ATXN2L fusion protein Ag20581
Full Name ataxin 2-like
Calculated molecular weight 1062 aa, 112 kDa
Observed molecular weight ~140 kDa
GenBank accession numberBC082760
Gene symbol ATXN2L
Gene ID (NCBI) 11273
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATXN2L antibody 24822-1-APDownload protocol
IHC protocol for ATXN2L antibody 24822-1-APDownload protocol
IP protocol for ATXN2L antibody 24822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

PMID: 30787271

Authors - Li Lin
  • KD Validated
View Article
mouseWB

Int J Mol Sci

Mid-Gestation lethality of Atxn2l-Ablated Mice.

Authors - Jana Key
View Article
humanWB

ACS Chem Biol

Deorphanizing Caspase-3 and Caspase-9 Substrates In and Out of Apoptosis with Deep Substrate Profiling.

Authors - Luam E Araya
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Sahil (Verified Customer) (06-21-2019)

I have checked others too and this works perfectly fine. If needed again would definitely buy it.

  • Applications: Western Blot,