ATXN2L PolyClonal antibody

Name: ATXN2L Rabbit PolyClonal antibody
Brand: Proteintech
SKU: 24822-1-AP
Rating: 5.00 (1 reviews)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 24822-1-AP

Synonyms

A2D, A2LG, A2LP, A2RP, Ataxin 2 domain protein, ataxin 2 like, Ataxin 2 like protein, Ataxin 2 related protein, ATXN2L

WB analysis of Jurkat using 24822-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 24822-1-AP (ATXN2L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 24822-1-AP
IP Result of anti-ATXN2L (IP:24822-1-AP, 4ug; Detection:24822-1-AP 1:2000) with HeLa cells lysate 2400ug.
IHC staining of human tonsillitis using 24822-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24822-1-AP (ATXN2L Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 24822-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24822-1-AP (ATXN2L Antibody) at dilution of 1:50 (under 40x lens).

Cat No. 24822-1-AP

Freight/Packing: -

Country/Region

Size/Concentration

Quantity

Positive WB detected in	Jurkat cells, mouse thymus tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human tonsillitis tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

KD/KO	See 1 publications below
WB	See 2 publications below

The immunogen of 24822-1-AP is ATXN2L Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	PolyClonal
Type	Antibody
Immunogen	ATXN2L fusion protein Ag20581
Full Name	ataxin 2-like
Calculated molecular weight	1062 aa, 112 kDa
Observed molecular weight	~140 kDa
GenBank accession number	BC082760
Gene symbol	ATXN2L
Gene ID (NCBI)	11273
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Product Specific Protocols
WB protocol for ATXN2L antibody 24822-1-AP	Download protocol
IHC protocol for ATXN2L antibody 24822-1-AP	Download protocol
IP protocol for ATXN2L antibody 24822-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Cell Death Dis PMID: 30787271 Authors - Li Lin KD Validated View Article
mouse	WB	Int J Mol Sci Mid-Gestation lethality of Atxn2l-Ablated Mice. Authors - Jana Key View Article

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Sahil (Verified Customer) (06-21-2019)

I have checked others too and this works perfectly fine. If needed again would definitely buy it.