|Positive WB detected in||Jurkat cells, mouse thymus tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human tonsillitis tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 24822-1-AP is ATXN2L Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATXN2L fusion protein Ag20581|
|Full Name||ataxin 2-like|
|Calculated molecular weight||1062 aa, 112 kDa|
|Observed molecular weight||~140 kDa|
|GenBank accession number||BC082760|
|Gene ID (NCBI)||11273|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cell Death Dis
Int J Mol Sci
Mid-Gestation lethality of Atxn2l-Ablated Mice.
ACS Chem Biol
Deorphanizing Caspase-3 and Caspase-9 Substrates In and Out of Apoptosis with Deep Substrate Profiling.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sahil (Verified Customer) (06-21-2019)
I have checked others too and this works perfectly fine. If needed again would definitely buy it.