Validation Data Gallery
|Positive WB detected in
|Jurkat cells, HeLa cells, MKN-45 cells
|Positive IP detected in
|Positive IHC detected in
|human tonsillitis tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:1000-1:8000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
24822-1-AP targets ATXN2L in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|ATXN2L fusion protein Ag20581
|Calculated molecular weight
|1062 aa, 112 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
ATXN2L is a paralog of Ataxin-2 (ATXN2) but without abnormal polyQ expended track, which is conserved in most of the ATXNs and drives the pathogenesis of neurodegeneration. It was reported that ATXN2L was widely expressed in immortalized cell lines, and ATXN2L-JAK2 fusion was found in CD4-positive T-cell lymphoma.
|Product Specific Protocols
|WB protocol for ATXN2L antibody 24822-1-AP
|IHC protocol for ATXN2L antibody 24822-1-AP
|IF protocol for ATXN2L antibody 24822-1-AP
|IP protocol for ATXN2L antibody 24822-1-AP
|Click here to view our Standard Protocols
Circadian clocks are modulated by compartmentalized oscillating translation
Int J Mol Sci
Mid-Gestation lethality of Atxn2l-Ablated Mice.
ACS Chem Biol
Deorphanizing Caspase-3 and Caspase-9 Substrates In and Out of Apoptosis with Deep Substrate Profiling.
Jatrorrhizine ameliorates Schwann cell myelination via inhibiting HDAC3 ability to recruit Atxn2l for regulating the NRG1-ErbB2-PI3K-AKT pathway in diabetic peripheral neuropathy mice
Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules
Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sahil (Verified Customer) (06-21-2019)
I have checked others too and this works perfectly fine. If needed again would definitely buy it.