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ATXN2L PolyClonal antibody
ATXN2L PolyClonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC
Conjugate
Unconjugated
Cat no : 24822-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, mouse thymus tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human tonsillitis tissue, human testis tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 3 publications below |
Product Information
The immunogen of 24822-1-AP is ATXN2L Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | PolyClonal |
Type | Antibody |
Immunogen | ATXN2L fusion protein Ag20581 |
Full Name | ataxin 2-like |
Calculated molecular weight | 1062 aa, 112 kDa |
Observed molecular weight | ~140 kDa |
GenBank accession number | BC082760 |
Gene symbol | ATXN2L |
Gene ID (NCBI) | 11273 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ATXN2L antibody 24822-1-AP | Download protocol |
IHC protocol for ATXN2L antibody 24822-1-AP | Download protocol |
IP protocol for ATXN2L antibody 24822-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
ACS Chem Biol Deorphanizing Caspase-3 and Caspase-9 Substrates In and Out of Apoptosis with Deep Substrate Profiling. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sahil (Verified Customer) (06-21-2019) | I have checked others too and this works perfectly fine. If needed again would definitely buy it.
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