• Featured Product
  • KD/KO Validated

ATXN2L PolyClonal antibody

ATXN2L PolyClonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC

Conjugate

Unconjugated

Cat no : 24822-1-AP

Synonyms

A2D, A2LG, A2LP, A2RP, Ataxin 2 domain protein, ataxin 2 like, Ataxin 2 like protein, Ataxin 2 related protein, ATXN2L



Tested Applications

Positive WB detected inJurkat cells, mouse thymus tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman tonsillitis tissue, human testis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24822-1-AP is ATXN2L Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class PolyClonal
Type Antibody
Immunogen ATXN2L fusion protein Ag20581
Full Name ataxin 2-like
Calculated molecular weight 1062 aa, 112 kDa
Observed molecular weight ~140 kDa
GenBank accession numberBC082760
Gene symbol ATXN2L
Gene ID (NCBI) 11273
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATXN2L antibody 24822-1-APDownload protocol
IHC protocol for ATXN2L antibody 24822-1-APDownload protocol
IP protocol for ATXN2L antibody 24822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

PMID: 30787271

Authors - Li Lin
  • KD Validated
mouseWB

Int J Mol Sci

Mid-Gestation lethality of Atxn2l-Ablated Mice.

Authors - Jana Key
humanWB

ACS Chem Biol

Deorphanizing Caspase-3 and Caspase-9 Substrates In and Out of Apoptosis with Deep Substrate Profiling.

Authors - Luam E Araya

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sahil (Verified Customer) (06-21-2019)

I have checked others too and this works perfectly fine. If needed again would definitely buy it.

  • Applications: Western Blot,