AUH Polyclonal antibody

AUH Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, RIP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17079-1-AP

Synonyms

AUH

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Validation Data Gallery

• 

  WB analysis of human brain using 17079-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17079-1-AP (AUH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 17079-1-AP

  IP result of anti-AUH (IP:17079-1-AP, 4ug; Detection:17079-1-AP 1:300) with HeLa cells lysate 2800 ug.

• 

  IHC staining of human brain using 17079-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17079-1-AP (AUH antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17079-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17079-1-AP (AUH antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• RIP: See 1 publications below

Product Information

17079-1-AP targets AUH in WB, IP, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AUH fusion protein Ag11265
• Full Name: AU RNA binding protein/enoyl-Coenzyme A hydratase
• Calculated Molecular Weight: 310 aa, 33 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC020722
• Gene Symbol: AUH
• Gene ID (NCBI): 549
• RRID: AB_2061316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	RIP	Int J Biol Macromol; m5C modification of LINC00324 promotes angiogenesis in glioma through CBX3/VEGFR2 pathway; Authors - Aini Pan