AUH Polyclonal antibody

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• AUH Polyclonal antibody (17079-1-AP)

• KD/KO Validated

AUH Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 17079-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, RIP, ELISA

Synonyms

3-MG-CoA hydratase, AU-binding protein/enoyl-CoA hydratase, AU-specific RNA-binding enoyl-CoA hydratase, EC:4.2.1.18, EC:4.2.1.56

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  WB analysis of human brain using 17079-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17079-1-AP (AUH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 17079-1-AP

  WB result of AUH antibody (17079-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-AUH transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IP experiment of HeLa using 17079-1-AP

  IP result of anti-AUH (IP:17079-1-AP, 4ug; Detection:17079-1-AP 1:300) with HeLa cells lysate 2800 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 17079-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17079-1-AP (AUH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 17079-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17079-1-AP (AUH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human brain tissue, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17079-1-AP targets AUH in WB, IHC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11265

  Product name: Recombinant human AUH protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-310 aa of BC020722

  Sequence: MAAAVAAAPGALGSLHAGGARLVAACSAWLCPGLRLPGSLAGRRAGPAIWAQGWVPAAGGPAPKRGYSSEMKTEDELRVRHLEEENRGIVVLGINRAYGKNSLSKNLIKMLSKAVDALKSDKKVRTIIIRSEVPGIFCAANLPVPTIAAIDGLALGGGLELALACDIRVAASSAKMGLVETKLAIIPGGGGTQRLPRAIGMSLAKELIFSARVLDGKEAKAVGLISHVLEQNQEGDAAYRKALDLAREFLPQGPVAMRVAKLAINQGMEVDLVTGLAIEEACYAQTIPTKDRLEGLLAFKEKRPPRYKGE

• Full Name: AU RNA binding protein/enoyl-Coenzyme A hydratase
• Calculated Molecular Weight: 310 aa, 33 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC020722
• Gene Symbol: AUH
• Gene ID (NCBI): 549
• RRID: AB_2061316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13825
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The AUH Polyclonal antibody (Cat# 17079-1-AP) has 2 citations. One appears in Nature Communications (2026), studying leucine catabolism and BAT thermogenesis in mice via WB. The other is in International Journal of Biological Macromolecules (2023), investigating m5C RNA modification and glioma angiogenesis in human samples via RIP. Research fields span metabolic regulation and epigenetic RNA modification in cancer.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Commun; Leucine catabolic enzyme AUH regulates BAT thermogenesis via PPARγ HMGylation and RNA-binding function in male mice; Authors - Haizhou Jiang
human	RIP	Int J Biol Macromol; m5C modification of LINC00324 promotes angiogenesis in glioma through CBX3/VEGFR2 pathway; Authors - Aini Pan