AURKA Recombinant antibody

AURKA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230087F7

Cat no : 82906-2-RR

Synonyms

Aurora A, AURK, AURA, ARK-1, AIRK1



Tested Applications

Positive WB detected inK-562 cells, MCF-7 cells, HeLa cells, NCCIT cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82906-2-RR targets AURKA in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AURKA fusion protein Ag0362
Full Name aurora kinase A
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46-52 kDa
GenBank Accession NumberBC002499
Gene Symbol AURKA
Gene ID (NCBI) 6790
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

Protocols

Product Specific Protocols
WB protocol for AURKA antibody 82906-2-RRDownload protocol
IF protocol for AURKA antibody 82906-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols