Tested Applications
| Positive WB detected in | HeLa cells, mouse thymus tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60098-1-Ig targets Aurora B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7532 Product name: Recombinant human AURKB protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-371 aa of BC013300 Sequence: QIQLFAGGRESSSALDPSSPPPFSLRMAQKENSYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILTRRHFTIDDFEIGRPLGKGKFGNVYLAREKKSHFIVALKVLFKSQIEKEGVEHQLRREIEIQAHLHHPNILRLYNYFYDRRRIYLILEYAPRGELYKELQKSCTFDEQRTATIMEELADALMYCHGKKVIHRDIKPENLLLGLKGELKIADFGWSVHAPSLRRKTMCGTLDYLPPEMIEGRMHNEKVDLWCIGVLCYELLVGNPPFESASHNETYRRIVKVDLKFPASVPMGAQDLISKLLRHNPSERLPLAQVSAHPWVRANSRRVLPPSALQSVA Predict reactive species |
| Full Name | aurora kinase B |
| Calculated Molecular Weight | 39.2 kDa |
| Observed Molecular Weight | 39-45 kDa |
| GenBank Accession Number | BC013300 |
| Gene Symbol | AURKB |
| Gene ID (NCBI) | 9212 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96GD4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Aurora B(AURKB) is also named as aurora kinase B, AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5 and belongs to the Aurora subfamily. Aurora kinase B is a chromosomal passenger serine/threonine protein kinase that regulates accurate chromosomal segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachments, and regulation of the mitotic checkpoint(PMID:18311747). Expression levels of AURKB are increased in the anaplastic thyroid carcinoma cell lines ARO, Cal 62, and KAT-4(PMID:15562011).
Publications
What published studies show
Aurora B Monoclonal antibody (3H1), catalog number 60098-1-Ig, has 4 total citations. These appear in Gastroenterology, FEBS J, Human Molecular Genetics, and PLoS One. The associated research fields include cancer cell proliferation and Hippo signaling, mitotic spindle function in lung cancer, postnatal cardiac development in spinal muscular atrophy models, and pancreatic cancer molecular targets. All cited applications are Western blot (WB) in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Gastroenterology Overexpression of Rac GTPase Activating Protein 1 Contributes to Proliferation of Cancer Cells by Reducing Hippo Signaling to Promote Cytokinesis. | ||
FEBS J YAP co-localizes with the mitotic spindle and midbody to safeguard mitotic division in lung-cancer cells | ||
Hum Mol Genet Downregulation of Survivin contributes to cell-cycle arrest during postnatal cardiac development in a severe spinal muscular atrophy mouse model. | ||
PLoS One Anti-pancreatic cancer deliverables from sea: first-hand evidence on the efficacy, molecular targets and mode of action for multifarious polyphenols from five different brown-algae. |
