AUTS2 Polyclonal antibody

AUTS2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 25001-1-AP

Synonyms

AUTS2, FBRSL2, KIAA0442

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Validation Data Gallery

• 

  WB analysis of C6 using 25001-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 25001-1-AP (AUTS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 25001-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25001-1-AP (AUTS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: C6 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 3 publications below
• ChIP: See 1 publications below

Product Information

The immunogen of 25001-1-AP is AUTS2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AUTS2 fusion protein Ag21853
• Full Name: autism susceptibility candidate 2
• Calculated Molecular Weight: 139 kDa
• Observed Molecular Weight: 70 kDa, 130-140 kDa
• GenBank Accession Number: BC064693
• Gene Symbol: AUTS2
• Gene ID (NCBI): 26053
• RRID: AB_2879840
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AUTS2 also named as FBRSL2 or autism susceptibility candidate 2 is a 1259 amino acid protein, which belongs to the AUTS2 family. AUTS2 is highly expressed in brain, kidney and skeletal muscle. AUTS2 exists as four isoforms produced by alternative splicing events and is encoded by a gene located on human chromosome 7. The molecular weight of AUTS2 isoform is 65-70 kDa in the brain ( PMID:23332918). The functional role of AUTS2 is not well known, although some studies have identified a putative role in transcriptional regulation during neuronal development.

Publications

Species	Application	Title
human	IF	Front Neurol; Tranylcypromine Causes Neurotoxicity and Represses BHC110/LSD1 in Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoids Model.; Authors - Jing Huang
human	IF	Neuroscience; PMID: 30599272; Authors - Fangkun Liu
mouse		Mol Cell; Functional Landscape of PCGF Proteins Reveals Both RING1A/B-Dependent-and RING1A/B-Independent-Specific Activities.; Authors - Andrea Scelfo
mouse	WB	Exp Neurol; Auts2 regulated autism-like behavior, glucose metabolism and oxidative stress in mice; Authors - Min Liu
human	WB,IF,ChIP	Nucleic Acids Res; CRISPR screening uncovers nucleolar RPL22 as a heterochromatin destabilizer and senescence driver; Authors - Hong-Yu Li