Tested Applications
| Positive WB detected in | HeLa cells, HT-1080 cells, HEK-293T cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16541-1-AP targets AXIN1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9858 Product name: Recombinant human AXIN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC044648 Sequence: MNIQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDPRPASYSFCSGKGVGIKGETSTATPRRSDLDLGYEPEGSASPTPPYLKWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACTGFRKLEPCDSNEEKRLKLARAIYRKYILDNNGIVSRQTKPATKSFIKGCIMKQLIDPAMFDQAQTEIQATMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGISGYLPTLNEDEEWKCDQDMDEDDGRDAAPPGRLPQKLLLETAAPRVSSSRRYSEGREFRYGSWREPVNPYYVNAGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQ Predict reactive species |
| Full Name | axin 1 |
| Calculated Molecular Weight | 826aa,92 kDa; 862aa,95 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC044648 |
| Gene Symbol | AXIN1 |
| Gene ID (NCBI) | 8312 |
| RRID | AB_2062409 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15169 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Axis inhibition protein1 (AXIN1), also called AXIN, together with AXIN2 are multidomain scaffold proteins that negatively regulate Wnt signaling. AXIN1 is likely to function as a tumor suppressor. Under UV irradiation, AXIN1-HIPK2-TP53 complex forms. The complex also controls cell growth, apoptosis and development. Like AXIN2, AXIN1 undergoes poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2 followed by unbiquitination by RNF146 which leads to its degradation and subsequent activation of Wnt signaling. Its deubiquitination by USP34 is important for nuclear accumulation during Wnt signaling. Recent researches find that CircAXIN1 encodes a novel protein, AXIN1-295aa, which shows at around 40-55 kDa by Western Blot. AXIN1-295aa functions as an oncogenic protein, activating the Wnt signaling pathway to promote GC tumorigenesis and progression, suggesting a potential therapeutic target for GC. Proteintech's AXIN1 antibody 16541-1-AP is a rabbit polyclonal antibdy raised against the N-terminus of human AXIN1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for AXIN1 antibody 16541-1-AP | Download protocol |
| WB protocol for AXIN1 antibody 16541-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
AXIN1 Polyclonal antibody (Cat# 16541-1-AP) has 24 citations across 23 journals, including Signal Transduction and Targeted Therapy, Communications Biology, International Journal of Molecular Sciences, Cellular Oncology, Frontiers in Oncology, FEBS Letters, and Journal of Traditional Chinese Medicine. Research fields span Wnt/β-catenin signaling, oncology (pancreatic, colorectal, hepatocellular, lung, cervical, gastric, glioblastoma), fibrosis (pulmonary, endometrial, oral), stem cell biology, and pharmacology.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther B-lymphoid tyrosine kinase-mediated FAM83A phosphorylation elevates pancreatic tumorigenesis through interacting with β-catenin | ||
Signal Transduct Target Ther Serine/threonine/tyrosine kinase 1 drives pancreatic carcinogenesis via GSK3β sequestration-mediated Wnt/β-catenin pathway hyperactivation. | ||
Int J Oncol Long non‑coding RNA 01614 hyperactivates WNT/β‑catenin signaling to promote pancreatic cancer progression by suppressing GSK‑3β | ||
Cancer Cell Int E3 ubiquitin ligase BTBD3 inhibits tumorigenesis of colorectal cancer by regulating the TYRO3/Wnt/β-catenin signaling axis | ||
Fundam Res O-GlcNAcylation of YTHDF2 antagonizes ERK-dependent phosphorylation and inhibits lung carcinoma. | ||
Biochem Pharmacol Corylifol A alleviates rheumatoid arthritis by targeting HSP90α and suppressing Wnt/β-catenin signaling. |
