|Positive WB detected in||HT-1080 cells, HEK-293T cells|
|Positive IHC detected in||human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16541-1-AP targets AXIN1 in WB, IHC, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||AXIN1 fusion protein Ag9858|
|Full Name||axin 1|
|Calculated molecular weight||826aa,92 kDa; 862aa,95 kDa|
|Observed molecular weight||100-110 kDa|
|GenBank accession number||BC044648|
|Gene ID (NCBI)||8312|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Axis inhibition protein1 (AXIN1), also called AXIN, together with AXIN2 are multidomain scaffold proteins that negatively regulate Wnt signaling. AXIN1 is likely to function as a tumor suppressor. Under UV irradiation, AXIN1-HIPK2-TP53 complex forms. The complex also controls cell growth, apoptosis and development. Like AXIN2, AXIN1 undergoes poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2 followed by unbiquitination by RNF146 which leads to its degradation and subsequent activation of Wnt signaling. Its deubiquitination by USP34 is important for nuclear accumulation during Wnt signaling. Proteintech's AXIN1 antibody 16541-1-AP is a rabbit polyclonal antibdy raised against the N-terminus of human AXIN1.
Targeting TRIM54/Axin1/β-Catenin Axis Prohibits Proliferation and Metastasis in Hepatocellular Carcinoma.
Cancer Gene Ther
Stabilizing and upregulating Axin with tankyrase inhibitor reverses 5-fluorouracil chemoresistance and proliferation by targeting the WNT/caveolin-1 axis in colorectal cancer cells.
Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.
Exp Cell Res
The transcript ENST00000444125 of lncRNA LINC01503 promotes cancer stem cell properties of glioblastoma cells via reducing FBXW1 mediated GLI2 degradation.
Exosomes derived from mineralizing osteoblasts promote ST2 cell osteogenic differentiation by alteration of microRNA expression.
Dig Dis Sci
HMGA2 elicits EMT by activating the Wnt/β-catenin pathway in gastric cancer.