AXIN2 Monoclonal antibody, PBS Only

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  WB analysis using 67458-1-Ig (same clone as 67458-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67458-1-Ig (AXIN2 antibody) at dilution of 1:4800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67458-1-PBS in a different storage buffer formulation.

AXIN2 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 67458-1-PBS

Clone No.1A11A4

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

Axin-2, Axil, axin 2, Axin like protein, Axin-like protein

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Product Information

67458-1-PBS targets AXIN2 in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AXIN2 fusion protein Ag29291
• Full Name: axin 2
• Calculated Molecular Weight: 843 aa, 94 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC006295
• Gene Symbol: AXIN2
• Gene ID (NCBI): 8313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9Y2T1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Axis inhibition protein2 (AXIN2), also known as Coductin or Axil, is a multidomain scaffold protein that negatively regulate Wnt signaling. AXIN2 can directly interact with beta-catenin and GSK3B. AXIN2 has a number of phosphorylation sites, and also can undergo poly(ADP-ribosy)lation by tankyrase TNKS and TNKS2. Poly(ADP-ribosy)lated AXIN2 then get unbiquitinated by RNF146 and leads to its degradation and subsequent activation of Wnt signaling. AXIN2 is localized in the cytoplasm and its mutation is involved in colorectal cancer.