Tested Applications
| Positive WB detected in | human plasma, MCF-7 cells |
| Positive IHC detected in | mouse liver tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13399-1-AP targets Zinc Alpha 2 Glycoprotein in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4232 Product name: Recombinant human AZGP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-298 aa of BC033830 Sequence: MVRMVPVLLSLLLLLGPAVPQENQDGRYSLTYIYTGLSKHVEDVPAFQALGSLNDLQFFRYNSKDRKSQPMGLWRQVEGMEDWKQDSQLQKAREDIFMETLKDIVEYYNDSNGSHVLQGRFGCEIENNRSSGAFWKYYYDGKDYIEFNKEIPAWVPFDPAAQITKQKWEAEPVYVQRAKAYLEEECPATLRKYLKYSKNILDRQDPPSVVVTSHQAPGEKKKLKCLAYDFYPGKIDVHWTRAGEVQEPELRGDVLHNGNGTYQSWVVVAVPPQDTAPYSCHVQHSSLAQPLVVPWEAS Predict reactive species |
| Full Name | alpha-2-glycoprotein 1, zinc-binding |
| Calculated Molecular Weight | 298 aa, 34 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC033830 |
| Gene Symbol | Zinc Alpha 2 Glycoprotein |
| Gene ID (NCBI) | 563 |
| RRID | AB_2062582 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P25311 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Zinc-alpha-2-glycoprotein (AZGP1) is a 41-kDa soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Zinc Alpha 2 Glycoprotein antibody 13399-1-AP | Download protocol |
| IHC protocol for Zinc Alpha 2 Glycoprotein antibody 13399-1-AP | Download protocol |
| WB protocol for Zinc Alpha 2 Glycoprotein antibody 13399-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-AZGP1 (Zinc-α2-glycoprotein) antibody (Cat# 13399-1-AP) has 7 citations published in Sci Adv, Cell Mol Biol Lett, J Transl Med, Invest Ophthalmol Vis Sci, Obesity, Int J Cancer, and Proteome Sci. Research fields span cystic fibrosis airway dysfunction, prostate cancer metastasis and PSA recurrence, hepatocellular carcinoma prognosis, subretinal fibrosis and EMT, adipose tissue insulin sensitivity, and urine proteome analysis. Applications include IHC, WB, and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Sci Adv Mucus concentration-dependent biophysical abnormalities unify submucosal gland and superficial airway dysfunction in cystic fibrosis. | ||
Cell Mol Biol Lett Methylation-induced silencing of AZGP1 enhances prostate cancer metastasis by stimulating tumoral glycolysis. | ||
J Transl Med Decreased expression of zinc-alpha2-glycoprotein in hepatocellular carcinoma associates with poor prognosis. | ||
Invest Ophthalmol Vis Sci AZGP1 Attenuates Subretinal Fibrosis and Inhibits Epithelial-Mesenchymal Transition by Blocking the PI3K/AKT Signaling Pathway | ||
Int J Cancer Reduced AZGP1 expression is an independent predictor of early PSA recurrence and associated with ERG fusion positive and PTEN deleted prostate cancers. |
