AZI1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86809-3-RR (same clone as 86809-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86809-3-RR (AZI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86809-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 86809-3-RR (same clone as 86809-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86809-3-RR (AZI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86809-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86809-3-RR (same clone as 86809-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AZI1 antibody (86809-3-RR, Clone: 251839D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86809-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86809-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86809-3-RR against Human AZI1 were performed. The affinity constant is 0.704 nM.

AZI1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86809-3-PBS

Clone No.251839D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

5 azacytidine induced 1, 5-azacytidine-induced protein 1, AZ1, CEP131, KIAA1118

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Product Information

86809-3-PBS targets AZI1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag22699

  Product name: Recombinant human AZI1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 730-1080 aa of BC136578

  Sequence: HEAELLQSDERASQRCLRQAEELREQLEREKEALGQQERERARQRFQQHLEQEQWALQQQRQRLYSEVAEERERLGQQAARQRAELEELRQQLEESSSALTRALRAEFEKGREEQERRHQMELNTLKQQLELERQAWEAGRTRKEEAWLLNREQELREEIRKGRDKEIELVIHRLEADMALAKEESEKAAESRIKRLRDKYEAELSELEQSERKLQERCSELKGQLGEAEGENLRLQGLVRQKERALEDAQAVNEQLSSERSNLAQVIRQEFEDRLAASEEETRQAKAELATLQARQQLELEEVHRRVKTALARKEEAVSSLRTQHEAAVKRADHLEELLEQHRRPTPSTK

• Full Name: 5-azacytidine induced 1
• Calculated Molecular Weight: 1083 aa, 122 kDa
• Observed Molecular Weight: 117-130 kDa
• GenBank Accession Number: BC136578
• Gene Symbol: AZI1
• Gene ID (NCBI): 22994
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UPN4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

AZI1, also named as CEP131 and KIAA1118, may play a role in spermatogenesis. It is located at the ciliary base and required for cilium formation.