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Trichostatin A treated NIH/3T3 cells and untreated cells were subjected to SDS PAGE followed by western blot with 84551-1-RR (Acetyl-Histone H2B (Lys120) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Trichostatin A treated NIH/3T3 cells and untreated cells were subjected to SDS PAGE followed by western blot with 84551-1-RR (Acetyl-Histone H2B (Lys120) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control.
IF Staining of HeLa using 84551-1-RR
Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H2B (Lys120) antibody (84551-1-RR, Clone: 241556E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H2B (Lys120) antibody (84551-1-RR, Clone: 241556E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC experiment of NIH/3T3 using 84551-1-RR
1x10^6 Trichostatin A treated NIH/3T3 cells were intracellularly stained with 0.13 ug Acetyl-Histone H2B (Lys120) Recombinant monoclonal antibody (84551-1-RR, Clone:241556E7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
1x10^6 Trichostatin A treated NIH/3T3 cells were intracellularly stained with 0.13 ug Acetyl-Histone H2B (Lys120) Recombinant monoclonal antibody (84551-1-RR, Clone:241556E7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).
Dot Blot experiment of peptide using 84551-1-RR
Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2B (Lys120) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2B (Lys120) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.
ChIP experiment of HeLa using 84551-1-RR
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2B (Lys120) (84551-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2B (Lys120) (84551-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).
Protocols
Product Specific Protocols
FC protocol for Acetyl-Histone H2B (Lys120) antibody 84551-1-RR
This validation data has been generated in-house and reviewed by Proteintech scientists.
Find out more on our antibody validation policy
WB Figures
WB analysis of NIH/3T3 using 84551-1-RR
Trichostatin A treated NIH/3T3 cells and untreated cells were subjected to SDS PAGE followed by western blot with 84551-1-RR (Acetyl-Histone H2B (Lys120) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control.
IF/ICC Figures
IF Staining of HeLa using 84551-1-RR
Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H2B (Lys120) antibody (84551-1-RR, Clone: 241556E7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC (INTRA) Figures
FC experiment of NIH/3T3 using 84551-1-RR
1x10^6 Trichostatin A treated NIH/3T3 cells were intracellularly stained with 0.13 ug Acetyl-Histone H2B (Lys120) Recombinant monoclonal antibody (84551-1-RR, Clone:241556E7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).
DOT BLOT Figures
Dot Blot experiment of peptide using 84551-1-RR
Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2B (Lys120) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.
CHIP-QPCR Figures
ChIP experiment of HeLa using 84551-1-RR
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2B (Lys120) (84551-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Acetyl-Histone H2B (Lys120) Recombinant antibody
Catalog Number
84551-1-RR
Citations
-
Dilutions
WB : 1:500-1:2000 IF/ICC : 1:500-1:2000 FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension DOT BLOT : 1:10-1:100 CHIP-QPCR : 1:10-1:100
Applications
WB, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
