Acetyl-Histone H3 (Lys23) Recombinant monoclonal antibody, PBS Only (82838-2-PBS)
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IHC staining of mouse testis using 82838-2-RR (same clone as 82838-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (HIST1H3A antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (HIST1H3A antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 82838-2-RR (same clone as 82838-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (Acetyl-Histone H3 (Lys23) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (Acetyl-Histone H3 (Lys23) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.
IHC staining of rat testis using 82838-2-RR (same clone as 82838-2-PBS)
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 82838-2-RR (Acetyl-Histone H3 (Lys23) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 82838-2-RR (Acetyl-Histone H3 (Lys23) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.
IF Staining of HeLa using 82838-2-RR (same clone as 82838-2-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H3 (Lys23) antibody (82838-2-RR, Clone: 241137E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H3 (Lys23) antibody (82838-2-RR, Clone: 241137E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.
Dot Blot experiment of peptide using 82838-2-RR (same clone as 82838-2-PBS)
Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys23) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.
Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys23) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.
ChIP experiment of HeLa using 82838-2-RR (same clone as 82838-2-PBS)
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys23) (82838-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys23) (82838-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.
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IHC staining of mouse testis using 82838-2-RR (same clone as 82838-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (HIST1H3A antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 82838-2-RR (same clone as 82838-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (Acetyl-Histone H3 (Lys23) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.
IHC staining of rat testis using 82838-2-RR (same clone as 82838-2-PBS)
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 82838-2-RR (Acetyl-Histone H3 (Lys23) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.
IF/ICC Figures
IF Staining of HeLa using 82838-2-RR (same clone as 82838-2-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H3 (Lys23) antibody (82838-2-RR, Clone: 241137E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.
DOT BLOT Figures
Dot Blot experiment of peptide using 82838-2-RR (same clone as 82838-2-PBS)
Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys23) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.
CHIP-QPCR Figures
ChIP experiment of HeLa using 82838-2-RR (same clone as 82838-2-PBS)
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys23) (82838-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Acetyl-Histone H3 (Lys23) Recombinant antibody
Catalog Number
82838-2-PBS
Citations
-
Dilutions
Applications
IHC, IF/ICC, Dot Blot, Indirect ELISA, ChIP-qPCR
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
