Acetyl-Histone H3 (Lys56) Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• Acetyl-Histone H3 (Lys56) Recombinant monoclonal antibody (87676-2-RR)

Acetyl-Histone H3 (Lys56) Uni-rAb^® Recombinant Antibody for FC (Intra), Dot Blot, ELISA, ChIP-qPCR

Cat No. 87676-2-RR

Clone No.252356B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), Dot Blot, ELISA, ChIP-qPCR

Synonyms

Ac Histone H3, Ac Histone H3 (Lys56), Ac Histone H3 Lys56, Ac Histone H3-Lys56, Acetyl Histone

• 

  FC experiment of NIH/3T3 using 87676-2-RR

  1x10^6 Trichostatin A treated NIH/3T3 cells were intracellularly stained with 0.13 ug Acetyl-Histone H3 (Lys56) Recombinant monoclonal antibody (87676-2-RR, Clone:252356B12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Dot Blot experiment of / using 87676-2-RR

  Dot blot analysis was used to confirm the specificity of 87676-2-RR Acetyl-Histone H3 (Lys56) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/mL.The amount of peptide (μg/mL) spotted is indicated next to each row.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  ChIP experiment of HeLa using 87676-2-RR

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys56) (87676-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive FC (Intra) detected in: Trichostatin A treated NIH/3T3 cells
• Positive Dot Blot detected in: / /
• Positive ChIP-qPCR detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
• DOT BLOT: DOT BLOT : 1:500-1:2000
• CHIP-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87676-2-RR targets Acetyl-Histone H3 (Lys56) in FC (Intra), Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H3a
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• RRID: AB_3745690
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P68431
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.