ChromoTek Actin-Chromobody® plasmid (TagGFP2)

DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagGFP2.

Vector type

Mammalian expression vector

Promoter

Constitutive CMV IE

Reporter

TagGFP2

Epitope

Actin

Publications

17

Cat no : acg

Synonyms

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Validation Data Gallery

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  Actin visualization in HeLa, PC3, U2OS, MEF, LNCaP, and BHK cells using Actin-Chromobody: Fluorescent microscopy images of cells transfected with Actin-Chromobody; green: TagGFP2, red: TagRFP (Evrogen), and blue: DAPI counterstain.

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Product Information

DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagGFP2.

• Applications: Live cell imaging
• Specificity: Actin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii
• Vector type: Mammalian expression vector
• Promoter: Constitutive CMV IE
• Reporter: TagGFP2
• Codon usage: Mammalian
• Selection: Kan/Neo
• Encoded Nanobody/ VHH: Monoclonal anti-Actin single domain antibody (sdAb) fragment
• Sequence: With the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to Actin fused to TagRFP or TagGFP2, as well as the full vector sequence.
• Microscopy techniques: Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED
• Transfection: Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
• Storage: Shipped at ambient temperature. Store at -20 °C

Publications

Application	Title
	Front Synaptic Neurosci; Interrogating Synaptic Architecture: Approaches for Labeling Organelles and Cytoskeleton Components.; Authors - Sofiia Reshetniak
IP	Methods Mol Biol; Visualization and Quantitative Analysis of the Actin Cytoskeleton Upon B Cell Activation.; Authors - Vid Šuštar
	Methods Cell Biol; Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos.; Authors - Catherine S Schrankel
	Cell Mol Bioeng; Characterization of 3D Printed Stretching Devices for Imaging Force Transmission in Live-Cells.; Authors - Carl R Mayer
	Methods; In vivo STED microscopy: A roadmap to nanoscale imaging in the living mouse.; Authors - Heinz Steffens
	Sci Rep; In vivo mouse and live cell STED microscopy of neuronal actin plasticity using far-red emitting fluorescent proteins.; Authors - Waja Wegner