ChromoTek Actin-Chromobody® plasmid (TagGFP2)

DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagGFP2.

Specificity

Actin

Applications

IF, Live cell imaging

Vector type

Mammalian expression vector

Reporter

TagGFP2

Publications

30

Cat no : acg

Synonyms

Actin Chromobody, Chromobody

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Validation Data Gallery

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Product Information

DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagGFP2.

• Description: The Actin-Chromobody allows imaging of actin dynamics in real-time in living cells.

  • Easily transfect your cells and non-invasively visualize actin cytoskeleton in real-time

  • Target and trace microfilaments in your cell line of interest

  • Use Actin-Chromobody as a marker of cellular morphology and as a probe for cellular motility

  • Actin-Chromobody has no notable effect cell viability or migration

• Applications: Live cell imaging; Visualizing actin dynamics; Actin as biomarker or control
• Specificity: Actin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii
• Reporter: TagGFP2
• Vector type: Mammalian expression vector
• Promoter: Constitutive CMV IE
• Codon usage: Mammalian
• Selection: Kan/Neo
• Nuclear localization signal (NLS): No
• Sequence: With the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to Actin fused to TagRFP or TagGFP2, as well as the full vector sequence.
• Transfection: Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
• Microscopy techniques: Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED
• Storage Condition: Shipped at ambient temperature. Store at -20 °C

Publications

Application	Title
	Nat Methods; Actin chromobody imaging reveals sub-organellar actin dynamics.; Authors - Cara R Schiavon
IF	Nat Commun; Optogenetic regulation of endogenous proteins.; Authors - Taras A Redchuk
WB	Nat Commun; Role of the nuclear membrane protein Emerin in front-rear polarity of the nucleus.; Authors - Paulina Nastały
IF	J Exp Med; IKKα controls ATG16L1 degradation to prevent ER stress during inflammation.; Authors - Michaela A Diamanti
	Dev Cell; Mechanism of Long-Range Chromosome Motion Triggered by Gene Activation.; Authors - Anqi Wang
IF	EMBO J; Initiation of DNA replication requires actin dynamics and formin activity.; Authors - Nikolaos Parisis