ChromoTek Actin-Chromobody® plasmid (TagGFP2)
DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagGFP2.
Vector type
Mammalian expression vector
Promoter
Constitutive CMV IE
Reporter
TagGFP2
Epitope
Actin
Cat no : acg
Synonyms
Validation Data Gallery
Product Information
DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagGFP2.
Applications | Live cell imaging |
Specificity | Actin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii |
Vector type | Mammalian expression vector |
Promoter | Constitutive CMV IE |
Reporter | TagGFP2 |
Codon usage | Mammalian |
Selection | Kan/Neo |
Encoded Nanobody/ VHH | Monoclonal anti-Actin single domain antibody (sdAb) fragment |
Sequence | With the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to Actin fused to TagRFP or TagGFP2, as well as the full vector sequence. |
Microscopy techniques | Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED |
Transfection | Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type. |
Storage | Shipped at ambient temperature. Store at -20 °C |
Documentation
Brochure |
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Product brochure (PDF) |
Datasheet |
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Datasheet Actin-Chromobody® TagGFP plasmid (PDF) |
Datasheet Actin-Chromobody® TagRFP plasmid (PDF) |
Datasheet Nuclear Actin-Chromobody® TagGFP plasmid (PDF) |
SDS |
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SDS Actin-Chromobody® TagGFP plasmid (PDF) |
SDS Nuclear Actin-Chromobody® TagGFP plasmid (PDF) |
Publications
Application | Title |
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Front Synaptic Neurosci Interrogating Synaptic Architecture: Approaches for Labeling Organelles and Cytoskeleton Components. | |
Methods Mol Biol Visualization and Quantitative Analysis of the Actin Cytoskeleton Upon B Cell Activation. | |
Methods Cell Biol Generation, expression and utilization of single-domain antibodies for in vivo protein localization and manipulation in sea urchin embryos. | |
Cell Mol Bioeng Characterization of 3D Printed Stretching Devices for Imaging Force Transmission in Live-Cells. | |
Sci Rep In vivo mouse and live cell STED microscopy of neuronal actin plasticity using far-red emitting fluorescent proteins. |