Adiponectin Polyclonal antibody

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  WB analysis using 30723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30723-1-AP (Adiponectin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brown adipose using 30723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 30723-1-AP (Adiponectin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brown adipose using 30723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 30723-1-AP (Adiponectin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Adiponectin Polyclonal Antibody for WB, IHC, ELISA

Cat No. 30723-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Adipoq, apM1, adipo, Acrp30

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Tested Applications

• Positive WB detected in: mouse adipose tissue, mouse serum
• Positive IHC detected in: mouse brown adipose tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30723-1-AP targets Adiponectin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: adiponectin, C1Q and collagen domain containing
• Calculated Molecular Weight: 27kd
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: NM_009605
• Gene Symbol: Adiponectin
• Gene ID (NCBI): 11450
• RRID: AB_3669755
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q60994
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.