Multi-rAb® Adiponectin receptor Multi-Recombinant antibody

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• Multi-rAb® Adiponectin receptor Multi-Recombinant antibody (RMX00098)

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  WB analysis using RMX00098

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00098 (Adiponectin receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using RMX00098

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using RMX00098 (Adiponectin receptor antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using RMX00098

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using RMX00098 (Adiponectin receptor antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using RMX00098

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Adiponectin receptor antibody (RMX00098) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Adiponectin receptor Multi-Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. RMX00098

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

ADIPOR2, ACDCR2, adiponectin receptor 2, Adiponectin receptor protein 2, ADIPOR1

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00098 targets Adiponectin receptor in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: adiponectin receptor 2
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC051858
• Gene Symbol: Adiponectin receptor
• Gene ID (NCBI): 79602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q86V24
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adiponectin is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Two receptors for adiponectin (ADIPOR1 and ADIPOR2) have been identified. They mediate increased AMPK, MAPK, and PPARA ligand activity in response to adiponectin. AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver (PMID: 12802337). The human ADIPOR2 gene maps to chromosome 12p13.31, and encodes a 386-amino acid protein with a molecular weight of 44 kDa. AdipoR2 may also exist as stable ~ 60 kDa dimers (PMID: 18842004). This antibody raised against 1-147aa of human AdipoR2 may cross-react with AdipoR1.