Albumin Recombinant monoclonal antibody, PBS Only

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• Albumin Recombinant monoclonal antibody, PBS Only (82783-6-PBS)

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  WB analysis using 82783-6-RR (same clone as 82783-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82783-6-RR (Albumin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

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  IHC staining of Human Hepatocellular cancer using 82783-6-RR (same clone as 82783-6-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 82783-6-RR (Albumin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 82783-6-RR (same clone as 82783-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82783-6-RR (Albumin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 82783-6-RR (same clone as 82783-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82783-6-RR (Albumin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

• 

  IHC staining of Human Hepatocellular cancer using 82783-6-RR (same clone as 82783-6-PBS)

  Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 82783-6-RR (Albumin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 82783-6-RR (same clone as 82783-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (82783-6-RR, Clone: 4K1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 82783-6-RR (same clone as 82783-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (82783-6-RR, Clone: 4K1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 82783-6-RR (same clone as 82783-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Albumin antibody (82783-6-RR, Clone: 4K1 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

Albumin Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 82783-6-PBS

Clone No.4K1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

ALB, 4K1, HSA, Serum albumin

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Product Information

82783-6-PBS targets Albumin in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9569

  Product name: Recombinant human Albumin protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 256-609 aa of BC034023

  Sequence: SKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL

• Full Name: albumin
• Calculated Molecular Weight: 609 aa, 69 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC034023
• Gene Symbol: Albumin
• Gene ID (NCBI): 213
• RRID: AB_3670549
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02768
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071)