Multi-rAb® Alix Multi-Recombinant antibody

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  WB analysis using RMX00081

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00081 (Alix antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using RMX00081

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using RMX00081 (Alix antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using RMX00081

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using RMX00081 (Alix antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using RMX00081

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Alix antibody (RMX00081) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Alix Multi-Recombinant Antibody for IHC, IF/ICC, ELISA

Cat No. RMX00081

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Synonyms

PDCD6IP, AIP1, ALG-2-interacting protein 1, ALG-2-interacting protein X, DRIP4

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1500-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00081 targets Alix in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Mixture

• Full Name: programmed cell death 6 interacting protein
• Calculated Molecular Weight: 868 aa, 96 kDa
• Observed Molecular Weight: 75-100 kDa
• GenBank Accession Number: BC020066
• Gene Symbol: Alix
• Gene ID (NCBI): 10015
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q8WUM4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium. The alix antibody recognizes an additional band of 75-80 kDa which has also been observed in cells and exosomes.