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Various lysates were subjected to SDS PAGE followed by western blot with RMX00081 (Alix antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with RMX00081 (Alix antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using RMX00081
IP result of anti-Alix (IP:RMX00081, 4ug; Detection:RMX00081 1:30000) with HeLa cells lysate 960 ug.
IP result of anti-Alix (IP:RMX00081, 4ug; Detection:RMX00081 1:30000) with HeLa cells lysate 960 ug.
IHC staining of human colon using RMX00081
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using RMX00081 (Alix antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using RMX00081 (Alix antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using RMX00081
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using RMX00081 (Alix antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using RMX00081 (Alix antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using RMX00081
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Alix antibody (RMX00081) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Alix antibody (RMX00081) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
HeLa cells, Jurkat cells, mouse liver tissue, rat liver tissue
Positive IP detected in
HeLa cells
Positive IHC detected in
human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HEK-293 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:1500-1:6000
Immunofluorescence (IF)/ICC
IF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
RMX00081 targets Alix in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium. The alix antibody recognizes an additional band of 75-80 kDa which has also been observed in cells and exosomes.
Various lysates were subjected to SDS PAGE followed by western blot with RMX00081 (Alix antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human colon using RMX00081
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using RMX00081 (Alix antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using RMX00081
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using RMX00081 (Alix antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HeLa using RMX00081
IP result of anti-Alix (IP:RMX00081, 4ug; Detection:RMX00081 1:30000) with HeLa cells lysate 960 ug.
IF/ICC Figures
IF Staining of HEK-293 using RMX00081
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Alix antibody (RMX00081) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Alix Multi-Recombinant antibody
Catalog Number
RMX00081
Citations
-
Dilutions
WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:1500-1:6000 IF/ICC : 1:250-1:1000
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
