Alkyl-DHAP synthase/AGPS Polyclonal antibody

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  WB analysis of HeLa using 21011-1-AP

  WB result of Alkyl-DHAP synthase/AGPS antibody (21011-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alkyl-DHAP synthase/AGPS transfected HeLa cells.

• 

  WB analysis using 21011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21011-1-AP (Alkyl-DHAP synthase/AGPS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21011-1-AP (Alkyl-DHAP synthase/AGPS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21011-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21011-1-AP (Alkyl-DHAP synthase/AGPS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

Alkyl-DHAP synthase/AGPS Polyclonal Antibody for WB, ELISA

Cat No. 21011-1-AP

Publications(2)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

ADAP S, ADAS, ADHAPS, ADPS, AGPS, ALDHPSY, Alkyl DHAP synthase, Alkyl-DHAP synthase/AGPS

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HepG2 cells, SW480 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

21011-1-AP targets Alkyl-DHAP synthase/AGPS in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15233

  Product name: Recombinant human AGPS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 309-658 aa of BC141820

  Sequence: TVGGWVSTRASGMKKNIYGNIEDLVVHIKMVTPRGIIEKSCQGPRMSTGPDIHHFIMGSEGTLGVITEATIKIRPVPEYQKYGSVAFPNFEQGVACLREIAKQRCAPASIRLMDNKQFQFGHALKPQVSSIFTSFLDGLKKFYITKFKGFDPNQLSVATLLFEGDREKVLQHEKQVYDIAAKFGGLAAGEDNGQRGYLLTYVIAYIRDLALEYYVLGESFETSAPWDRVVDLCRNVKERITRECKEKGVQFAPFSTCRVTQTYDAGACIYFYFAFNYRGISDPLTVFEQTEAAAREEILANGGSLSHHHGVGKLRKQWLKESISDVGFGMLKSVKEYVDPNNIFGNRNLL

• Full Name: alkylglycerone phosphate synthase
• Calculated Molecular Weight: 658 aa, 73 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC141820
• Gene Symbol: AGPS
• Gene ID (NCBI): 8540
• RRID: AB_2878789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00116
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB	Free Radic Biol Med; NEAT1/hsa-miR-372-3p axis participates in rapamycin-induced lipid metabolic disorder.; Authors - Guanghan Fan ; KD Validated
human	WB	Cell Chem Biol; Context-dependent regulation of ferroptosis sensitivity.; Authors - Leslie Magtanong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joshua (Verified Customer) (10-28-2022)

Antibody not specific, but AGPS protein was detected in MDA-MB-231 cell line

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF-7, MDA-MB-231