Alkyl-DHAP synthase/AGPS Polyclonal antibody

• Featured Product
• KD/KO Validated

Alkyl-DHAP synthase/AGPS Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 21011-1-AP

Synonyms

ADAP S, ADAS, ADHAPS, ADPS, AGPS, ALDHPSY, Alkyl DHAP synthase, Alkyl-DHAP synthase/AGPS

---

Validation Data Gallery

• 

  WB analysis of HeLa using 21011-1-AP

  WB result of Alkyl-DHAP synthase/AGPS antibody (21011-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alkyl-DHAP synthase/AGPS transfected HeLa cells.

• 

  WB analysis using 21011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21011-1-AP (Alkyl-DHAP synthase/AGPS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21011-1-AP (Alkyl-DHAP synthase/AGPS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21011-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21011-1-AP (Alkyl-DHAP synthase/AGPS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HepG2 cells, SW480 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

The immunogen of 21011-1-AP is Alkyl-DHAP synthase/AGPS Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Alkyl-DHAP synthase/AGPS fusion protein Ag15233
• Full Name: alkylglycerone phosphate synthase
• Calculated Molecular Weight: 658 aa, 73 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC141820
• Gene Symbol: AGPS
• Gene ID (NCBI): 8540
• RRID: AB_2878789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	Free Radic Biol Med; NEAT1/hsa-miR-372-3p axis participates in rapamycin-induced lipid metabolic disorder.; Authors - Guanghan Fan ; KD Validated
human	WB	Cell Chem Biol; Context-dependent regulation of ferroptosis sensitivity.; Authors - Leslie Magtanong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Joshua (Verified Customer) (10-28-2022)

Antibody not specific, but AGPS protein was detected in MDA-MB-231 cell line

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF-7, MDA-MB-231