Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, HepG2 cells, SW480 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21011-1-AP targets Alkyl-DHAP synthase/AGPS in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15233 Product name: Recombinant human AGPS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 309-658 aa of BC141820 Sequence: TVGGWVSTRASGMKKNIYGNIEDLVVHIKMVTPRGIIEKSCQGPRMSTGPDIHHFIMGSEGTLGVITEATIKIRPVPEYQKYGSVAFPNFEQGVACLREIAKQRCAPASIRLMDNKQFQFGHALKPQVSSIFTSFLDGLKKFYITKFKGFDPNQLSVATLLFEGDREKVLQHEKQVYDIAAKFGGLAAGEDNGQRGYLLTYVIAYIRDLALEYYVLGESFETSAPWDRVVDLCRNVKERITRECKEKGVQFAPFSTCRVTQTYDAGACIYFYFAFNYRGISDPLTVFEQTEAAAREEILANGGSLSHHHGVGKLRKQWLKESISDVGFGMLKSVKEYVDPNNIFGNRNLL Predict reactive species |
| Full Name | alkylglycerone phosphate synthase |
| Calculated Molecular Weight | 658 aa, 73 kDa |
| Observed Molecular Weight | 68-70 kDa |
| GenBank Accession Number | BC141820 |
| Gene Symbol | AGPS |
| Gene ID (NCBI) | 8540 |
| RRID | AB_2878789 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00116 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Alkyl-DHAP synthase/AGPS antibody 21011-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Alkyl-DHAP synthase/AGPS Polyclonal antibody (Cat# 21011-1-AP) has 3 citations. They appear in Cell Chemical Biology, EMBO Journal, and Free Radical Biology and Medicine. The associated research fields include ferroptosis regulation, peroxisome-derived ether lipid–mediated lysosomal exocytosis, and rapamycin-induced lipid metabolic disorder, all involving human (and one mouse) samples via Western blot.
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med NEAT1/hsa-miR-372-3p axis participates in rapamycin-induced lipid metabolic disorder.
|
Reviews
What customers say
Both reviews describe use in Western Blot at a 1:1000 dilution. One user validated a knockdown in HEK-293 lysates and rated the antibody 4/5, noting it was not highly specific but sufficient for their WB. The other user tested it on MCF-7 and MDA-MB-231 cell lines, rating it 1/5 due to poor specificity, though the AGPS protein was still detected in MDA-MB-231. Overall, reviewers report that the antibody can detect its target but exhibits limited specificity, with mixed satisfaction depending on the application's stringency requirements.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hélène (Verified Customer) (01-14-2026) | I used the antibody to validate a KD. It was not very specific but still worked well enough for a western blot along with a ladder.
|
Joshua (Verified Customer) (10-28-2022) | Antibody not specific, but AGPS protein was detected in MDA-MB-231 cell line
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