Alpl Polyclonal antibody

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• Alpl Polyclonal antibody (33076-1-AP)

Alpl Polyclonal Antibody for WB, ELISA

Cat No. 33076-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

Akp2, Akp-2, Alkaline phosphatase 2, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase, tissue-nonspecific isozyme

• 

  WB analysis using 33076-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33076-1-AP (Alpl antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, mouse kidney tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33076-1-AP targets Alpl in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg2714

  Product name: Recombinant Mouse ALPL protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 18-501 aa of NM_001287172.1

  Sequence: FVPEKERDPSYWRQQAQETLKNALKLQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYPKNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNLKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDQAIGKAGAMTSQKDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVDGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCIGANLDHCAWAG

• Full Name: alkaline phosphatase, liver/bone/kidney
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: NM_001287172.1
• Gene Symbol: Alpl
• Gene ID (NCBI): 11647
• RRID: AB_3742845
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P09242
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is expressed in multiple tissues including liver, bone, and kidney, rather than being restricted to a single tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Front Pharmacol; Exosomes derived from Panax notoginseng promote osteogenic differentiation of rBMSCs via the PI3K/AKT signaling pathway.; Authors - Nan Wu
rat	WB	J Mol Histol; Alpha-2-macroglobulin attenuates glucocorticoid-induced osteonecrosis of the femoral head by enhancing osteogenesis and antioxidant defense via the Akt/Nrf2 signaling pathway.; Authors - Qingjian He
		Biomaterials; A ZIF90-tungsten-based nanoparticle hydrogel system with antibacterial and antioxidant properties promotes periodontal bone regeneration by modulating the ROS/Plagl1/Wnt pathway.; Authors - Zhenyuan Yu
mouse	WB	Braz J Med Biol Res; MMP9 regulates osteogenesis and MMP2 expression through the TGF-β1/SMAD2/3 signaling pathway in lipopolysaccharide-induced osteoblasts.; Authors - Lei Li
mouse	WB	Phytomedicine; Shouhui Tongbian capsule promotes calcium absorption by regulating gut microbiota and protects against osteoporosis.; Authors - Mingyu Xu
mouse, rat	WB	Bioact Mater; Chiral Fe(3)O(4)/GelMA hydrogels regulate the osteoimmune microenvironment via Itgb3-mediated macrophage polarization to combat peri-implantitis.; Authors - Diwen Shi