Tested Applications
| Positive WB detected in | HeLa cells, mouse kidney tissue, rat kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
Product Information
33076-1-AP targets Alpl in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Eg2714 Product name: Recombinant Mouse ALPL protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 18-501 aa of NM_001287172.1 Sequence: FVPEKERDPSYWRQQAQETLKNALKLQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNTGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERTRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIKDIDVIMGGGRKYMYPKNRTDVEYELDEKARGTRLDGLDLISIWKSFKPRHKHSHYVWNRTELLALDPSRVDYLLGLFEPGDMQYELNRNNLTDPSLSEMVEVALRILTKNLKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDQAIGKAGAMTSQKDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMVSDTDKKPFTAILYGNGPGYKVVDGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFAKGPMAHLLHGVHEQNYIPHVMAYASCIGANLDHCAWAG Predict reactive species |
| Full Name | alkaline phosphatase, liver/bone/kidney |
| Calculated Molecular Weight | 58 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | NM_001287172.1 |
| Gene Symbol | Alpl |
| Gene ID (NCBI) | 11647 |
| RRID | AB_3742845 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P09242 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Alpl antibody 33076-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Pharmacol Exosomes derived from Panax notoginseng promote osteogenic differentiation of rBMSCs via the PI3K/AKT signaling pathway. | ||
Bioact Mater Chiral Fe(3)O(4)/GelMA hydrogels regulate the osteoimmune microenvironment via Itgb3-mediated macrophage polarization to combat peri-implantitis. | ||
Int J Biol Macromol Near-infrared responsive nanozyme-carboxymethyl chitosan/chondroitin sulfate composite hydrogel: Multicomponent synergy optimizes bone defect microenvironment to enhance repair. | ||
Phytomedicine Shouhui Tongbian capsule promotes calcium absorption by regulating gut microbiota and protects against osteoporosis. | ||
Adv Healthc Mater Calcium-Polyphenol Functionalized Black Phosphorus Nanosheet System for Osteoporosis Therapy via Macrophage Reprogramming and Bone Microenvironment Remodeling. | ||
Braz J Med Biol Res MMP9 regulates osteogenesis and MMP2 expression through the TGF-β1/SMAD2/3 signaling pathway in lipopolysaccharide-induced osteoblasts. |
