Angiotensinogen/AGT Recombinant monoclonal antibody

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  WB analysis using 86134-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86134-1-RR (Agt antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86134-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86134-1-RR (Agt antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Angiotensinogen/AGT Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86134-1-RR

Clone No.250570F8

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

Agt, Serpina8

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat liver tissue, mouse brain tissue, mouse lung tissue, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86134-1-RR targets Angiotensinogen/AGT in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2763

  Product name: Recombinant Mouse Angiotensinogen protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 25-477 aa of N/A

  Sequence: DRVYIHPFHLLYHNKSTCAQLENPSVETLPESTFEPVPIQAKTSPVNEKTLHDQLVLAAEKLEDEDRKRAAQVAMIANFVGFRMYKMLNEAGSGASGAILSPPALFGTLVSFYLGSLDPTASQLQTLLDVPVKEGDCTSRLDGHKVLAALRAVQGLLVTQGGSSSQTPLLQSIMVGLFTAPGFRLKHSFVQSLALFTPALFPRSLDLSTDPVLATEKINRFIKAVTGWKMNLPLEGVSTDSTLLFNTYVHFQGTMRGFSQLPGVHEFWVDNSISVSVPMISGTGNFQHWSDAQNNFSVTCVPLGERATLLLIQPHCTSDLDRVEALIFRNDLLTWIENPPPRAIRLTLPQLEIRGSYNLQDLLAEDKLPTLLGAEANLSNIGDTNPRVGEVLNSILLELKAGEEEQPTTSVQQPGSPEALDVTLSSPFLFAIYEQDSGTLHFLGRVNNPQSVV

• Full Name: angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52-55 kDa
• GenBank Accession Number: N/A
• Gene Symbol: Agt
• Gene ID (NCBI): 11606
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11859
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.