Angiotensinogen/AGT Polyclonal antibody, PBS Only

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• Angiotensinogen/AGT Polyclonal antibody, PBS Only (11992-1-PBS)

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  WB analysis using 11992-1-AP (same clone as 11992-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11992-1-AP (Angiotensinogen antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11992-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 11992-1-AP (same clone as 11992-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11992-1-AP (Angiotensinogen antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11992-1-PBS in a different storage buffer formulation.

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  IP experiment of human plasma using 11992-1-AP (same clone as 11992-1-PBS)

  IP result of anti-Angiotensinogen (IP:11992-1-AP, 4ug; Detection:11992-1-AP 1:1000) with human plasma lysate 1600 ug. This data was developed using the same antibody clone with 11992-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11992-1-AP (same clone as 11992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11992-1-AP (Angiotensinogen antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11992-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 11992-1-AP (same clone as 11992-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11992-1-AP (Angiotensinogen antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11992-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11992-1-AP (same clone as 11992-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Angiotensinogen antibody (11992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11992-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 11992-1-AP (same clone as 11992-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Angiotensinogen Polyclonal antibody (11992-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 11992-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Angiotensinogen/AGT Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 11992-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

AGT, Angiotensinogen, Serpin A8, SERPINA8, Alpha 1 antiproteinase

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Product Information

11992-1-PBS targets Angiotensinogen/AGT in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2632

  Product name: Recombinant human AGT protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 136-485 aa of BC011519

  Sequence: PTAVFGTLASLYLGALDHTADRLQAILGVPWKDKNCTSRLDAHKVLSALQAVQGLLVAQGRADSQAQLLLSTVVGVFTAPGLHLKQPFVQGLALYTPVVLPRSLDFTELDVAAEKIDRFMQAVTGWKTGCSLTGASVDSTLAFNTYVHFQGKMKGFSLLAEPQEFWVDNSTSVSVPMLSGMGTFQHWSDIQDNFSVTQVSFTESACLLLIQPHYASDLDKVEGLTFQQNSLNWMKKLSPRTIHLTMPQLVLQGSYDLQDLLAQAELPAILHTELNLQKLSNDRIRVGEVLNSIFFELEADEREPTESTQQLNKPEVLEVTLNRPFLFAVYDQSATALHFLGRVANPLSTA

• Full Name: angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• Calculated Molecular Weight: 485 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC011519
• Gene Symbol: AGT
• Gene ID (NCBI): 183
• RRID: AB_2225452
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P01019
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene also have been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.