Angptl4 Polyclonal antibody

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  WB analysis using 51109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51109-1-AP (Angptl4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 51109-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 51109-1-AP (Angptl4 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 51109-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 51109-1-AP (Angptl4 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 51109-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 51109-1-AP (Angptl4 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 51109-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 51109-1-AP (Angptl4 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 51109-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 51109-1-AP (Angptl4 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 51109-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 51109-1-AP (Angptl4 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Angptl4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 51109-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Hepatic fibrinogen/angiopoietin-related protein, ANGPTL4 N-terminal chain, ANGPTL4 C-terminal chain, Angiopoietin-related protein 4, Angiopoietin-like protein 4

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human kidney tissue, rat kidney tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

51109-1-AP targets Angptl4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Angptl4 fusion protein Ag0613
• Full Name: angiopoietin-like 4
• Calculated Molecular Weight: 405 aa, 45 kDa
• GenBank Accession Number: BC078944
• Gene Symbol: Angptl4
• Gene ID (NCBI): 362850
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6TMA8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. ANGPTL4 has three functional domains: the signal peptide, the coiled-coil (N-terminal chain, CCD), and the fibrinogen-like (C-terminal chain, FLD) domains. ANGPTL4 is a glycosylated secreted protein (fANGPTL4, 45-65 kDa). fANGPTL is then rapidly post-translationally cleaved into an N-terminal coiled-coil domain (nANGPTL4, ~ 15 kDa) and a C-terminal fibrinogen-like domain (cANGPTL4, ~ 35 kDa)(PMID: 37300585 ).