Anti-Human Annexin A1 Rabbit Recombinant Antibody

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• Anti-Human Annexin A1 Rabbit Recombinant Antibody (98737-2-RR)

Annexin A1 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98737-2-RR

Clone No.251332F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

ANXA1, Annexin 1, Annexin Ac2-26, Annexin I, Annexin-1

• 

  FC experiment of Jurkat using 98737-2-RR

  1x10^6 Staurosporine-treated Jurkat cells were surface stained with 0.25 ug Anti-Human Annexin A1 Rabbit RecAb (98737-2-RR, Clone: 251332F5) or 0.25 ug Isotype Control, Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005) and 7-AAD. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of K-562 using 98737-2-RR

  1x10^6 K-562 cells (left) or HEK-293T cells (right) were surface stained with 0.25 ug Anti-Human Annexin A1 Rabbit RecAb (98737-2-RR, Clone: 251332F5) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: Staurosporine treated Jurkat cells, K-562 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98737-2-RR targets Annexin A1 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg4503

  Product name: recombinant human Annexin A1 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 1-346 aa of NM_000700.3

  Sequence: MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN

• Full Name: annexin A1
• Calculated Molecular Weight: 39kDa
• GenBank Accession Number: NM_000700.3
• Gene Symbol: Annexin A1
• Gene ID (NCBI): 301
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04083
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Annexin A1 (ANXA1), also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.