Annexin VI Recombinant monoclonal antibody

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• Annexin VI Recombinant monoclonal antibody (87752-4-RR)

Annexin VI Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87752-4-RR

Clone No.253305F12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Annexin A6, ANXA6, 67 kDa calelectrin, Annexin 6, Annexin-6

• 

  WB analysis using 87752-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87752-4-RR (Annexin VI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 87752-4-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Annexin VI antibody (87752-4-RR, Clone: 253305F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, Raji cells, HepG2 cells, mouse brain tissue, human skeletal muscle tissue, rat liver tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87752-4-RR targets Annexin VI in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3263

  Product name: Recombinant human ANXA6 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 324-674 aa of BC017046

  Sequence: DDDAAGQFFPEAAQVAYQMWELSAVARVELKGTVRPANDFNPDADAKALRKAMKGLGTDEDTIIDIITHRSNVQRQQIRQTFKSHFGRDLMTDLKSEISGDLARLILGLMMPPAHYDAKQLKKAMEGAGTDEKALIEILATRTNAEIRAINEAYKEDYHKSLEDALSSDTSGHFRRILISLATGHREEGGENLDQAREDAQVAAEILEIADTPSGDKTSLETRFMTILCTRSYPHLRRVFQEFIKMTNYDVEHTIKKEMSGDVRDAFVAIVQSVKNKPLFFADKLYKSMKGAGTDEKTLTRIMVSRSEIDLLNIRREFIEKYDKSLHQAIEGDTSGDFLKALLALCGGED

• Full Name: annexin A6
• Calculated Molecular Weight: 673 aa, 76 kDa
• Observed Molecular Weight: 67-72 kDa
• GenBank Accession Number: BC017046
• Gene Symbol: Annexin VI
• Gene ID (NCBI): 309
• RRID: AB_3745731
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08133
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.