Various lysates were subjected to SDS PAGE followed by western blot with 31454-1-AP (Apoe antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 31454-1-AP (Apoe antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 31454-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 31454-1-AP (Apoe antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 31454-1-AP (Apoe antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of rat cerebellum using 31454-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 31454-1-AP (Apoe antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 31454-1-AP (Apoe antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 31454-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 31454-1-AP (Apoe antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 31454-1-AP (Apoe antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 31454-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Apoe antibody (31454-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Apoe antibody (31454-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
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rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:300-1:1200
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
31454-1-AP targets Apoe in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Apolipoprotein E (Apoe) is a core component of plasma lipoproteins that participates in diverse biological processes, including plasma lipoprotein metabolism, intracellular cholesterol utilization, cell growth, immunoregulation, and neuronal growth and repair (PMID: 17854398). Mouse apoE, like apoE4, contains the equivalent of Arg-112 and Glu-255, but lacks the critical Arg-61 equivalent (it contains Thr-61). Thus, mouse apoE does not display apoE4 domain interaction (PMID: 11553788).
Various lysates were subjected to SDS PAGE followed by western blot with 31454-1-AP (Apoe antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 31454-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 31454-1-AP (Apoe antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of rat cerebellum using 31454-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 31454-1-AP (Apoe antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 31454-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 31454-1-AP (Apoe antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HepG2 using 31454-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Apoe antibody (31454-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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