Tested Applications
| Positive WB detected in | U-251 cells, BV-2 cells, C2C12 cells, mouse brain tissue, mouse skeletal muscle tissue, mouse cerebellum tissue, rat brain tissue, pig cerebellum tissue |
| Positive IHC detected in | rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31454-1-AP targets APOE in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Eg1093 Product name: Recombinant Mouse APOE protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 19-311 aa of NM_001305819.1 Sequence: EGEPEVTDQLEWQSNQPWEQALNRFWDYLRWVQTLSDQVQEELQSSQVTQELTALMEDTMTEVKAYKKELEEQLGPVAEETRARLGKEVQAAQARLGADMEDLRNRLGQYRNEVHTMLGQSTEEIRARLSTHLRKMRKRLMRDAEDLQKRLAVYKAGAREGAERGVSAIRERLGPLVEQGRQRTANLGAGAAQPLRDRAQAFGDRIRGRLEEVGNQARDRLEEVREHMEEVRSKMEEQTQQIRLQAEIFQARLKGWFEPIVEDMHRQWANLMEKIQASVATNPIITPVAQENQ Predict reactive species |
| Full Name | Apolipoprotein E |
| Calculated Molecular Weight | 36kDa |
| Observed Molecular Weight | 31~36 kDa |
| GenBank Accession Number | NM_001305819.1 |
| Gene Symbol | Apoe |
| Gene ID (NCBI) | 11816 |
| RRID | AB_3669993 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P08226 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Apolipoprotein E (Apoe) is a core component of plasma lipoproteins that participates in diverse biological processes, including plasma lipoprotein metabolism, intracellular cholesterol utilization, cell growth, immunoregulation, and neuronal growth and repair (PMID: 17854398). Mouse apoE, like apoE4, contains the equivalent of Arg-112 and Glu-255, but lacks the critical Arg-61 equivalent (it contains Thr-61). Thus, mouse apoE does not display apoE4 domain interaction (PMID: 11553788).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for APOE antibody 31454-1-AP | Download protocol |
| IHC protocol for APOE antibody 31454-1-AP | Download protocol |
| WB protocol for APOE antibody 31454-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
Both reviewers gave this APOE antibody a 5-star rating for Western blotting. One user tested it on mouse brain tissue for an Alzheimer's disease study, reporting highly reproducible results with a clear, well-defined band at the expected 34–36 kDa range, and expressed strong satisfaction with intent to repurchase. The other user confirmed excellent performance on astrocyte conditioned medium samples. Overall, the antibody is praised for its specificity, reproducibility, and reliable band detection across different sample types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zakia (Verified Customer) (08-04-2026) | Excellent primary antibody for Western blotting. We tested this APOE antibody on mouse brain tissue samples for our Alzheimer's disease study. The results were highly reproducible, showing a clear, well-defined band right in the expected 34–36 kDa range. We are very satisfied and will be purchasing this product again.
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Andrea (Verified Customer) (07-24-2026) | It works very well by WB on astrocyte conditioned medium samples
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