Arginase-2 Recombinant antibody

• 

  WB analysis using 84808-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84808-5-RR (Arginase-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. Mouse liver tissue as a negative control.

Arginase-2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84808-5-RR

Clone No.242362A9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

ARG2, Arginase-2, mitochondrial, arginase, type II, Arginase II, ARG 2

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Tested Applications

• Positive WB detected in: U-251 cells, K-562 cells, Caco-2 cells,

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84808-5-RR targets Arginase-2 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Arginase-2 fusion protein Ag6609
• Full Name: arginase, type II
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC001350
• Gene Symbol: ARG2
• Gene ID (NCBI): 384
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P78540
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Arginase 2 is composed of 354 amino acid residues, including an NH2-terminal presequence for mitochondrial targeting and import. In the mitochondria, ornithine generated by Arginase 2 will give rise to glutamate via ornithine aminotransferase (OAT). Glutamate participates in several transamination reactions, including forming α-ketoglutarate (αKG) that may enter the TCA cycle and increase cycle intermediates and flux.Arginase 1 is mainly expressed in hepatocytes, and mice with a disruption of Arginase 1 gene die soon after birth. Arginase 2 is poorly expressed in hepatocytes, and most highly expressed in kidney, prostate, and immune cells such as monocyte/ macrophages. (PMID: 25234945,PMID: 27214549)