Arginase-2 Recombinant monoclonal antibody

• 

  WB analysis using 84808-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84808-5-RR (Arginase-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. Mouse liver tissue as a negative control.

• 

  WB analysis using 84808-5-RR

  Mouse kidney lysates were subjected to SDS PAGE followed by western blot with 84808-5-RR (Arginase-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 84808-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84808-5-RR (Arginase-2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 84808-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84808-5-RR against Human Arginase-2 were performed. The affinity constant is below 1 pM.

Arginase-2 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 84808-5-RR

Clone No.242362A9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

ARG2, 242362A9, ARG 2, Arginase 2, Arginase II

---

Tested Applications

• Positive WB detected in: U-251 cells, mouse kidney tissue, K-562 cells, Caco-2 cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84808-5-RR targets Arginase-2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6609

  Product name: Recombinant human ARG2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-354 aa of BC001350

  Sequence: MSLRGSLSRLLQTRVHSILKKSVHSVAVIGAPFSQGQKRKGVEHGPAAIREAGLMKRLSSLGCHLKDFGDLSFTPVPKDDLYNNLIVNPRSVGLANQELAEVVSRAVSDGYSCVTLGGDHSLAIGTISGHARHCPDLCVVWVDAHADINTPLTTSSGNLHGQPVSFLLRELQDKVPQLPGFSWIKPCISSASIVYIGLRDVDPPEHFILKNYDIQYFSMRDIDRLGIQKVMERTFDLLIGKRQRPIHLSFDIDAFDPTLAPATGTPVVGGLTYREGMYIAEEIHNTGLLSALDLVEVNPQLATSEEEAKTTANLAVDVIASSFGQTREGGHIVYDQLPTPSSPDESENQARVRI

• Full Name: arginase, type II
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC001350
• Gene Symbol: ARG2
• Gene ID (NCBI): 384
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P78540
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Arginase 2 is composed of 354 amino acid residues, including an NH2-terminal presequence for mitochondrial targeting and import. In the mitochondria, ornithine generated by Arginase 2 will give rise to glutamate via ornithine aminotransferase (OAT). Glutamate participates in several transamination reactions, including forming α-ketoglutarate (αKG) that may enter the TCA cycle and increase cycle intermediates and flux.Arginase 1 is mainly expressed in hepatocytes, and mice with a disruption of Arginase 1 gene die soon after birth. Arginase 2 is poorly expressed in hepatocytes, and most highly expressed in kidney, prostate, and immune cells such as monocyte/ macrophages. (PMID: 25234945,PMID: 27214549)