AXL Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 84659-1-RR (same clone as 84659-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84659-1-RR (Axl antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84659-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 84659-1-RR (same clone as 84659-1-PBS)

  WB result of AXL antibody (84659-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AXL transfected HeLa cells. This data was developed using the same antibody clone with 84659-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84659-1-RR (same clone as 84659-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AXL antibody (84659-1-RR, Clone: 242014B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84659-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

AXL Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 84659-1-PBS

Clone No.242014B9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

242014B9, EC:2.7.10.1, Tyrosine-protein kinase receptor UFO, UFO

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Product Information

84659-1-PBS targets AXL in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1618

  Product name: Recombinant Human AXL protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 33-449 aa of NM_021913.4

  Sequence: EESPFVGNPGNITGARGLTGTLRCQLQVQGEPPEVHWLRDGQILELADSTQTQVPLGEDEQDDWIVVSQLRITSLQLSDTGQYQCLVFLGHQTFVSQPGYVGLEGLPYFLEEPEDRTVAANTPFNLSCQAQGPPEPVDLLWLQDAVPLATAPGHGPQRSLHVPGLNKTSSFSCEAHNAKGVTTSRTATITVLPQQPRNLHLVSRQPTELEVAWTPGLSGIYPLTHCTLQAVLSDDGMGIQAGEPDPPEEPLTSQASVPPHQLRLGSLHPHTPYHIRVACTSSQGPSSWTHWLPVETPEGVPLGPPENISATRNGSQAFVHWQEPRAPLQGTLLGYRLAYQGQDTPEVLMDIGLRQEVTLELQGDGSVSNLTVCVAAYTAAGDGPWSLPVPLEAWRPGQAQPVHQLVKEPSTPAFSWP

• Full Name: AXL receptor tyrosine kinase
• Calculated Molecular Weight: 98kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: NM_021913.4
• Gene Symbol: AXL
• Gene ID (NCBI): 558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30530-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).