Beta-2-Microglobulin Monoclonal antibody

Beta-2-Microglobulin Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF, ELISA

Conjugate

Unconjugated

CloneNo.

1H3F4

Cat no : 68395-1-Ig

Synonyms

B2M, beta 2 microglobulin, HDCMA22P

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Validation Data Gallery

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  IF Staining of NCCIT using 68395-1-Ig

  Immunofluorescent analysis of NCCIT cells (non-fixation) using Beta-2-Microglobulin antibody (68395-1-Ig, Clone: 1H3F4 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of A431 using 68395-1-Ig

  Immunofluorescent analysis of A431 cells using Beta-2-Microglobulin antibody (68395-1-Ig, Clone: 1H3F4 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive IF detected in: NCCIT cells, A431 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68395-1-Ig targets Beta-2-Microglobulin in IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: beta-2-microglobulin
• Calculated Molecular Weight: 119 aa, 14 kDa
• Observed Molecular Weight: 12-14 kDa
• GenBank Accession Number: BC032589
• Gene Symbol: B2M
• Gene ID (NCBI): 567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases. This antibody can be used for staining living cells.