Beta-2-Microglobulin Recombinant monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP00673-3

  Sandwich ELISA standard curve of MP00673-3, human B2M Recombinant Matched Antibody Pair - PBS only. 83683-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4433. 83683-5-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

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  Sample test of MP00673-3

  Urine of eight individual healthy human donors was measured. The B2M concentration of detected samples was determined to be 202.46 ng/mL with a range of 67.46-352.77 ng/mL.

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  Sample test of MP00673-3

  Serum of eight individual healthy human donors was measured. The B2M concentration of detected samples was determined to be 223.48 ng/mL with a range of 111.44-289.89 ng/mL.

Beta-2-Microglobulin Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83683-1-PBS

Clone No.240660B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

B2M, beta 2 microglobulin, beta 2-Microglobulin, Beta-2-microglobulin form pI 5.3, CDABP0092

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Product Information

83683-1-PBS targets Beta-2-Microglobulin as part of a matched antibody pair:

MP00673-3: 83683-1-PBS capture and 83683-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4433

  Product name: Recombinant human B2M protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 22-119 aa of BC032589

  Sequence: QRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

• Full Name: beta-2-microglobulin
• Calculated Molecular Weight: 119 aa, 14 kDa
• GenBank Accession Number: BC032589
• Gene Symbol: B2M
• Gene ID (NCBI): 567
• ENSEMBL Gene ID: ENSG00000166710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61769
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.