Anti-Mouse Beta-2-Microglobulin Rabbit Recombinant Antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP02174-3

  Sandwich ELISA standard curve of MP02174-3, Mouse Beta-2-Microglobulin Recombinant Matched Antibody Pair - PBS only. 98442-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3086. 85871-5-PBS was HRP conjugated as the detection antibody. Range: 23.438-1500 pg/mL

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  Sample test of MP02174-3

  Urine of six mice was measured. The mouse Beta-2-Microglobulin concentration of detected samples was determined to be 49.78 ng/mL with a range of ND - 136.66 ng/mL.

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  Sample test of MP02174-3

  Serum of six mice was measured. The mouse Beta-2-Microglobulin concentration of detected samples was determined to be 94.41 µg/mL with a range of 79.57 - 105.38 µg/mL.

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  FC experiment of mouse splenocytes using 98442-2-RR (same clone as 98442-2-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse Beta-2-Microglobulin Rabbit RecAb (98442-2-RR, Clone: 250172F11) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red). Cells were not fixed. This data was developed using the same antibody clone with 98442-2-PBS in a different storage buffer formulation.

Beta-2-Microglobulin Uni-rAb^TM Recombinant Antibody for FC, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 98442-2-PBS

Clone No.250172F11

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

B2m, beta 2 microglobulin

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Product Information

98442-2-PBS targets Beta-2-Microglobulin in FC, Sandwich ELISA, Indirect ELISA, Sample test applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Beta-2-Microglobulin fusion protein Eg3086
• Full Name: beta-2 microglobulin
• Calculated Molecular Weight: 14 kDa
• GenBank Accession Number: NM_009735.3
• Gene Symbol: B2m
• Gene ID (NCBI): 12010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01887
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.