B3GALNT1 Polyclonal antibody

B3GALNT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22048-1-AP

Synonyms

b3Gal T3, B3GALNT1, B3GALT3, Beta 1,3 GalNAc T1, Beta 1,3 GalTase 3, Beta 3 Gx T3, beta3Gal T3, Beta3GalT3, galT3, Gb4Cer, GLCT3, GLOB, Globoside synthase, P1

---

Validation Data Gallery

• 

  WB analysis using 22048-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22048-1-AP (B3GALNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 22048-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 22048-1-AP (B3GALNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 22048-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22048-1-AP (B3GALNT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 22048-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22048-1-AP (B3GALNT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 22048-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22048-1-AP (B3GALNT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 22048-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22048-1-AP (B3GALNT1 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse placenta tissue, MCF-7 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human heart tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22048-1-AP targets B3GALNT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: B3GALNT1 fusion protein Ag16976
• Full Name: beta-1,3-N-acetylgalactosaminyltransferase 1 (globoside blood group)
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC047618
• Gene Symbol: B3GALNT1
• Gene ID (NCBI): 8706
• RRID: AB_11182184
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.