B3GALNT2 Polyclonal antibody

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  WB analysis of HEK-293 using 17142-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17142-1-AP (B3GALNT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 17142-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using B3GALNT2 antibody (17142-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

B3GALNT2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 17142-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Beta-1,3-GalNAc-T2, Beta-1,3-N-acetylgalactosaminyltransferase II, EC:2.4.1.313, UDP GalNAc:beta 1,3 N acetylgalactosaminyltransferase 2, UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 2

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

17142-1-AP targets B3GALNT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10907

  Product name: Recombinant human B3GALNT2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 167-500 aa of BC029564

  Sequence: NDVGFQRNITVKLYQAEQEEALFIARFSPPSCGVQVNKLWYKPVEQFILPESFEGTIVWESQDLHGLVSRNLHKVTVNDGGGVLRVITAGEGALPHEFLEGVEGVAGGFIYTIQEGDALLHNLHSRPQRLIDHIRNLHEEDALLKEESSIYDDIVFVDVVDTYRNVPAKLLNFYRWTVETTSFNLLLKTDDDCYIDLEAVFNRIVQKNLDGPNFWWGNFRLNWAVDRTGKWQELEYPSPAYPAFACGSGYVISKDIVKWLASNSGRLKTYQGEDVSMGIWMAAIGPKRYQDSLWLCEKTCETGMLSSPQYSPWELTELWKLKERCGDPCRCQAR

• Full Name: beta-1,3-N-acetylgalactosaminyltransferase 2
• Calculated Molecular Weight: 500 aa, 57 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC029564
• Gene Symbol: B3GALNT2
• Gene ID (NCBI): 148789
• RRID: AB_2063040
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NCR0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IHC	J Hematol Oncol; Increased B3GALNT2 in hepatocellular carcinoma promotes macrophage recruitment via reducing acetoacetate secretion and elevating MIF activity.; Authors - Tianxiao Yang