Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17142-1-AP targets B3GALNT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10907 Product name: Recombinant human B3GALNT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 167-500 aa of BC029564 Sequence: NDVGFQRNITVKLYQAEQEEALFIARFSPPSCGVQVNKLWYKPVEQFILPESFEGTIVWESQDLHGLVSRNLHKVTVNDGGGVLRVITAGEGALPHEFLEGVEGVAGGFIYTIQEGDALLHNLHSRPQRLIDHIRNLHEEDALLKEESSIYDDIVFVDVVDTYRNVPAKLLNFYRWTVETTSFNLLLKTDDDCYIDLEAVFNRIVQKNLDGPNFWWGNFRLNWAVDRTGKWQELEYPSPAYPAFACGSGYVISKDIVKWLASNSGRLKTYQGEDVSMGIWMAAIGPKRYQDSLWLCEKTCETGMLSSPQYSPWELTELWKLKERCGDPCRCQAR Predict reactive species |
| Full Name | beta-1,3-N-acetylgalactosaminyltransferase 2 |
| Calculated Molecular Weight | 500 aa, 57 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC029564 |
| Gene Symbol | B3GALNT2 |
| Gene ID (NCBI) | 148789 |
| RRID | AB_2063040 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NCR0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for B3GALNT2 antibody 17142-1-AP | Download protocol |
| WB protocol for B3GALNT2 antibody 17142-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The B3GALNT2 Polyclonal antibody (Cat# 17142-1-AP) has 2 citations. They appear in J Hematol Oncol and Mol Genet Metab. The first study investigated B3GALNT2's role in promoting macrophage recruitment in hepatocellular carcinoma, linking it to cancer biology and tumor microenvironment research. The second examined truncated N-glycans' effects on POMT1/POMT2 stability and O-mannosylation, relating to glycobiology and congenital disorders of glycosylation. Cited applications include WB and IHC, with reactivity confirmed in human and mouse samples.
| Species | Application | Title |
|---|---|---|
J Hematol Oncol Increased B3GALNT2 in hepatocellular carcinoma promotes macrophage recruitment via reducing acetoacetate secretion and elevating MIF activity. | ||
Mol Genet Metab Truncated N-glycans destabilize POMT1 and POMT2 but do not limit cellular O-mannosylation in HEK293 cells. |
