B3GALNT2 Polyclonal antibody

B3GALNT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17142-1-AP

Synonyms

B3GalNAc T2, B3GALNT2, Beta 1,3 GalNAc T2



Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17142-1-AP targets B3GALNT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B3GALNT2 fusion protein Ag10907
Full Name beta-1,3-N-acetylgalactosaminyltransferase 2
Calculated Molecular Weight 500 aa, 57 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC029564
Gene Symbol B3GALNT2
Gene ID (NCBI) 148789
RRIDAB_2063040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for B3GALNT2 antibody 17142-1-APDownload protocol
IHC protocol for B3GALNT2 antibody 17142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

J Hematol Oncol

Increased B3GALNT2 in hepatocellular carcinoma promotes macrophage recruitment via reducing acetoacetate secretion and elevating MIF activity.

Authors - Tianxiao Yang