Tested Applications
| Positive WB detected in | HT-29 cells, mouse small intestine tissue, rat small intestine tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33022-1-AP targets B3GALT5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37977 Product name: Recombinant human B3GALT5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 29-310 aa of BC104862 Sequence: NPFKEQSFVYKKDGNFLKLPDTDCRQTPPFLVLLVTSSHKQLAERMAIRQTWGKERTVKGKQLKTFFLLGTTSSAAETKEVDQESQRHGDIIQKDFLDVYYNLTLKTMMGIEWVHRFCPQAAFVMKTDSDMFINVDYLTELLLKKNRTTRFFTGFLKLNEFPIRQPFSKWFVSKSEYPWDRYPPFCSGTGYVFSGDVASQVYNVSKSVPYIKLEDVFVGLCLERLNIRLEELHSQPTFFPGGLRFSVCLFRRIVACHFIKPRTLLDYWQALENSRGEDCPPV Predict reactive species |
| Full Name | UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 |
| Calculated Molecular Weight | 310 aa, 36 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC104862 |
| Gene Symbol | B3GALT5 |
| Gene ID (NCBI) | 10317 |
| RRID | AB_3742824 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9Y2C3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
B3GALT5 (β-1,3-galactosyltransferase 5) is a key glycosyltransferase enzyme. It is responsible for catalyzing the synthesis of the terminal glycan structure, the sialyl Lewis X antigen. This antigen serves as an important ligand for selectin family proteins, mediating cell adhesion in both physiological and pathological processes. Its core function lies in regulating cell migration. In immunology, it influences lymphocyte homing; in oncology, its aberrant upregulation promotes the synthesis of sLe^x, leading to the adhesion of cancer cells to vascular endothelium via the "selectin-ligand" recognition system. This represents a critical initial step in tumor hematogenous metastasis, such as to the liver or lungs.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for B3GALT5 antibody 33022-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
